1ce0: Difference between revisions
New page: left|200px<br /> <applet load="1ce0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ce0, resolution 2.4Å" /> '''TRIMERIZATION SPECIF... |
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'''TRIMERIZATION SPECIFICITY IN HIV-1 GP41: ANALYSIS WITH A GCN4 LEUCINE ZIPPER MODEL'''<br /> | '''TRIMERIZATION SPECIFICITY IN HIV-1 GP41: ANALYSIS WITH A GCN4 LEUCINE ZIPPER MODEL'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1CE0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http:// | 1CE0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CE0 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: protein oligomerization]] | [[Category: protein oligomerization]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:35:20 2008'' | ||
Revision as of 16:35, 15 February 2008
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TRIMERIZATION SPECIFICITY IN HIV-1 GP41: ANALYSIS WITH A GCN4 LEUCINE ZIPPER MODEL
OverviewOverview
The envelope glycoprotein of human immunodeficiency virus type 1 (HIV-1), consists of a complex of two noncovalently associated subunits, gp120 and, gp41. Formation of gp120/gp41 oligomers is thought to be dependent on a, 4-3 hydrophobic (heptad) repeat located in the amino-terminal region of, the gp41 molecule. We have investigated the role of this heptad repeat in, determining the oligomeric structure of gp41 by introducing its buried, core residues into the first (a) and fourth (d) positions of the GCN4, leucine-zipper dimerization domain. The mutant peptides fold into, trimeric, helical structures, as shown by circular dichroism and, equilibrium sedimentation centrifugation. The 2.4 A resolution crystal, structure of one such trimer reveals a parallel three-stranded, alpha-helical coiled coil. Thus, the buried core residues from the gp41, heptad repeat direct trimer formation. We suggest that the conserved, amino-terminal heptad repeat within the gp41 ectodomain possesses, trimerization specificity.
About this StructureAbout this Structure
1CE0 is a Single protein structure of sequence from Human immunodeficiency virus 1. Full crystallographic information is available from OCA.
ReferenceReference
Trimerization specificity in HIV-1 gp41: analysis with a GCN4 leucine zipper model., Shu W, Ji H, Lu M, Biochemistry. 1999 Apr 27;38(17):5378-85. PMID:10220324
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