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==Crystal structure of a galactokinase from Bifidobacterium infantis in complex with ADP== | ==Crystal structure of a galactokinase from Bifidobacterium infantis in complex with ADP== | ||
<StructureSection load='6tep' size='340' side='right'caption='[[6tep]]' scene=''> | <StructureSection load='6tep' size='340' side='right'caption='[[6tep]], [[Resolution|resolution]] 1.45Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TEP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TEP FirstGlance]. <br> | <table><tr><td colspan='2'>[[6tep]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bifls Bifls]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TEP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TEP FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tep OCA], [http://pdbe.org/6tep PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tep RCSB], [http://www.ebi.ac.uk/pdbsum/6tep PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tep ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Blon_2062 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=391904 BIFLS])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tep OCA], [http://pdbe.org/6tep PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tep RCSB], [http://www.ebi.ac.uk/pdbsum/6tep PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tep ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Fluorinated sugar-1-phosphates are of emerging importance as intermediates in the chemical and biocatalytic synthesis of modified oligosaccharides, as well as probes for chemical biology. Here we present a systematic study of the activity of a wide range of anomeric sugar kinases (galacto- and N-acetylhexosamine kinases) against a panel of fluorinated monosaccharides, leading to the first examples of polyfluorinated substrates accepted by this class of enzymes. We have discovered four new N-acetylhexosamine kinases with a different substrate scope, thus expanding the number of homologs available in this subclass of kinases. Lastly, we have solved the crystal structure of a galactokinase in complex with 2-deoxy-2-fluorogalactose, giving insight into changes in the active site that may account for the specificity of the enzyme toward certain substrate analogs. | |||
Profiling Substrate Promiscuity of Wild-Type Sugar Kinases for Multi-fluorinated Monosaccharides.,Keenan T, Parmeggiani F, Malassis J, Fontenelle CQ, Vendeville JB, Offen W, Both P, Huang K, Marchesi A, Heyam A, Young C, Charnock SJ, Davies GJ, Linclau B, Flitsch SL, Fascione MA Cell Chem Biol. 2020 Jun 30. pii: S2451-9456(20)30228-2. doi:, 10.1016/j.chembiol.2020.06.005. PMID:32619452<ref>PMID:32619452</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6tep" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Bifls]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Both P]] | [[Category: Both, P]] | ||
[[Category: Charnock S]] | [[Category: Charnock, S]] | ||
[[Category: Davies | [[Category: Davies, G J]] | ||
[[Category: Fascione | [[Category: Fascione, M A]] | ||
[[Category: Flitsch | [[Category: Flitsch, S L]] | ||
[[Category: Fontenelle | [[Category: Fontenelle, C Q]] | ||
[[Category: Heyam A]] | [[Category: Heyam, A]] | ||
[[Category: Huang K]] | [[Category: Huang, K]] | ||
[[Category: Keenan T]] | [[Category: Keenan, T]] | ||
[[Category: Linclau B]] | [[Category: Linclau, B]] | ||
[[Category: Malassis J]] | [[Category: Malassis, J]] | ||
[[Category: Marchesi A]] | [[Category: Marchesi, A]] | ||
[[Category: Offen | [[Category: Offen, W A]] | ||
[[Category: Parmeggiani F]] | [[Category: Parmeggiani, F]] | ||
[[Category: Vendeville J]] | [[Category: Vendeville, J]] | ||
[[Category: Young C]] | [[Category: Young, C]] | ||
[[Category: Adp]] | |||
[[Category: Complex]] | |||
[[Category: Galactokinase]] | |||
[[Category: Transferase]] | |||
Revision as of 14:31, 22 July 2020
Crystal structure of a galactokinase from Bifidobacterium infantis in complex with ADPCrystal structure of a galactokinase from Bifidobacterium infantis in complex with ADP
Structural highlights
Publication Abstract from PubMedFluorinated sugar-1-phosphates are of emerging importance as intermediates in the chemical and biocatalytic synthesis of modified oligosaccharides, as well as probes for chemical biology. Here we present a systematic study of the activity of a wide range of anomeric sugar kinases (galacto- and N-acetylhexosamine kinases) against a panel of fluorinated monosaccharides, leading to the first examples of polyfluorinated substrates accepted by this class of enzymes. We have discovered four new N-acetylhexosamine kinases with a different substrate scope, thus expanding the number of homologs available in this subclass of kinases. Lastly, we have solved the crystal structure of a galactokinase in complex with 2-deoxy-2-fluorogalactose, giving insight into changes in the active site that may account for the specificity of the enzyme toward certain substrate analogs. Profiling Substrate Promiscuity of Wild-Type Sugar Kinases for Multi-fluorinated Monosaccharides.,Keenan T, Parmeggiani F, Malassis J, Fontenelle CQ, Vendeville JB, Offen W, Both P, Huang K, Marchesi A, Heyam A, Young C, Charnock SJ, Davies GJ, Linclau B, Flitsch SL, Fascione MA Cell Chem Biol. 2020 Jun 30. pii: S2451-9456(20)30228-2. doi:, 10.1016/j.chembiol.2020.06.005. PMID:32619452[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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