|
|
| Line 3: |
Line 3: |
| <SX load='5fkv' size='340' side='right' viewer='molstar' caption='[[5fkv]], [[Resolution|resolution]] 8.00Å' scene=''> | | <SX load='5fkv' size='340' side='right' viewer='molstar' caption='[[5fkv]], [[Resolution|resolution]] 8.00Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[5fkv]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FKV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FKV FirstGlance]. <br> | | <table><tr><td colspan='2'>[[5fkv]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FKV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FKV FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5fku|5fku]], [[5fkw|5fkw]]</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8Å</td></tr> |
| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fkv OCA], [https://pdbe.org/5fkv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fkv RCSB], [https://www.ebi.ac.uk/pdbsum/5fkv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fkv ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fkv OCA], [http://pdbe.org/5fkv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fkv RCSB], [http://www.ebi.ac.uk/pdbsum/5fkv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fkv ProSAT]</span></td></tr> | |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/DPO3X_ECOLI DPO3X_ECOLI]] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. Isoform tau: serves as a scaffold to help in the dimerization of the core complex. Isoform gamma: seems to interact with the delta subunit. to transfer the beta subunit on the DNA. [[http://www.uniprot.org/uniprot/DPO3A_ECOLI DPO3A_ECOLI]] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase. [[http://www.uniprot.org/uniprot/DPO3E_ECOLI DPO3E_ECOLI]] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease. [[http://www.uniprot.org/uniprot/DPO3B_ECOLI DPO3B_ECOLI]] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA. | | [https://www.uniprot.org/uniprot/DPO3A_ECOLI DPO3A_ECOLI] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase. |
| <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 26: |
Line 25: |
| __TOC__ | | __TOC__ |
| </SX> | | </SX> |
| [[Category: DNA-directed DNA polymerase]] | | [[Category: Escherichia coli K-12]] |
| [[Category: Ecoli]]
| |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Conrad, J]] | | [[Category: Synthetic construct]] |
| [[Category: Fernandez-Leiro, R]] | | [[Category: Conrad J]] |
| [[Category: Lamers, M H]] | | [[Category: Fernandez-Leiro R]] |
| [[Category: Scheres, S H.W]] | | [[Category: Lamers MH]] |
| [[Category: Dna polymerase iii alpha]]
| | [[Category: Scheres SHW]] |
| [[Category: Dna polymerase iii beta]]
| |
| [[Category: Dna polymerase iii epsilon]]
| |
| [[Category: Dna polymerase iii tau]]
| |
| [[Category: Dna replication]]
| |
| [[Category: Transferase]]
| |