1au7: Difference between revisions

Revision as of 17:36, 30 June 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:1au7.png

Template:STRUCTURE 1au7

PIT-1 MUTANT/DNA COMPLEXPIT-1 MUTANT/DNA COMPLEX

Template:ABSTRACT PUBMED 9009203

About this StructureAbout this Structure

1AU7 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

ReferenceReference

Structure of Pit-1 POU domain bound to DNA as a dimer: unexpected arrangement and flexibility., Jacobson EM, Li P, Leon-del-Rio A, Rosenfeld MG, Aggarwal AK, Genes Dev. 1997 Jan 15;11(2):198-212. PMID:9009203

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-'''PIT-1 MUTANT/DNA COMPLEX'''
+===PIT-1 MUTANT/DNA COMPLEX===
-==Overview==
+<!--
-Pit-1, a member of the POU domain family of transcription factors, characterized by a bipartite DNA-binding domain, serves critical developmental functions based on binding to diverse DNA elements in its target genes. Here we report a high resolution X-ray analysis of the Pit-1 POU domain bound to a DNA element as a homodimer. This analysis reveals that Pit-1 subdomains bind to perpendicular faces of the DNA, rather than opposite faces of the DNA as in Oct-1. This is accomplished by different spacing and orientation of the POU-specific domain. Contrary to previous predictions, the dimerization interface involves the carboxyl terminus of the DNA recognition helix of the homeodomain, which in an extended conformation interacts with specific residues at the amino terminus of helix alpha1 and in the loop between helices alpha3 and alpha4 of the POU-specific domain of the symmetry related monomer. These features suggest the molecular basis of disease-causing mutations in Pit-1 and provide potential basis for the flexible allostery between protein domains and DNA sites in the activation of target genes.
+The line below this paragraph, {{ABSTRACT_PUBMED_9009203}}, adds the Publication Abstract to the page
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 ==About this Structure==
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 [[Category: Pou domain]]
 [[Category: Transcription factor]]
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