6bbi: Difference between revisions
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<StructureSection load='6bbi' size='340' side='right'caption='[[6bbi]], [[Resolution|resolution]] 4.35Å' scene=''> | <StructureSection load='6bbi' size='340' side='right'caption='[[6bbi]], [[Resolution|resolution]] 4.35Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6bbi]] is a 3 chain structure with sequence from [ | <table><tr><td colspan='2'>[[6bbi]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BBI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BBI FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.35Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bbi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bbi OCA], [https://pdbe.org/6bbi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bbi RCSB], [https://www.ebi.ac.uk/pdbsum/6bbi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bbi ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/CRCM1_DROME CRCM1_DROME] Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores. Regulates transcription factor NFAT nuclear import.<ref>PMID:16582901</ref> <ref>PMID:16645049</ref> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Drosophila melanogaster]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Burstein | [[Category: Burstein S]] | ||
[[Category: Hou | [[Category: Hou X]] | ||
[[Category: Long | [[Category: Long SB]] | ||
Latest revision as of 17:37, 4 October 2023
The CRAC channel Orai in an unlatched-closed conformation; K163W loss-of-function mutation; P42212 crystal formThe CRAC channel Orai in an unlatched-closed conformation; K163W loss-of-function mutation; P42212 crystal form
Structural highlights
FunctionCRCM1_DROME Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores. Regulates transcription factor NFAT nuclear import.[1] [2] Publication Abstract from PubMedThe store-operated calcium (Ca(2+)) channel Orai governs Ca(2+) influx through the plasma membrane of many non-excitable cells in metazoans. The channel opens in response to the depletion of Ca(2+) stored in the endoplasmic reticulum (ER). Loss- and gain-of-function mutants of Orai cause disease. Our previous work revealed the structure of Orai with a closed pore. Here, using a gain-of-function mutation that constitutively activates the channel, we present an X-ray structure of Drosophila melanogaster Orai in an open conformation. Well-defined electron density maps reveal that the pore is dramatically dilated on its cytosolic side in comparison to the slender closed pore. Cations and anions bind in different regions of the open pore, informing mechanisms for ion permeation and Ca(2+) selectivity. Opening of the pore requires the release of cytosolic latches. Together with additional X-ray structures of an unlatched-but-closed conformation, we propose a sequence for store-operated activation. Structures reveal opening of the store-operated calcium channel Orai.,Hou X, Burstein SR, Long SB Elife. 2018 Aug 30;7. pii: 36758. doi: 10.7554/eLife.36758. PMID:30160233[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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