6uph: Difference between revisions

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<StructureSection load='6uph' size='340' side='right'caption='[[6uph]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
<StructureSection load='6uph' size='340' side='right'caption='[[6uph]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6uph]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UPH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UPH FirstGlance]. <br>
<table><tr><td colspan='2'>[[6uph]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/ ], [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast] and [http://en.wikipedia.org/wiki/Candida_sphaerica Candida sphaerica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UPH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UPH FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6uph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uph OCA], [http://pdbe.org/6uph PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uph RCSB], [http://www.ebi.ac.uk/pdbsum/6uph PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uph ProSAT]</span></td></tr>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CSE4, CSL2, YKL049C, YKL262 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), KLLA0_E08647g, KLLA0_E17601g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Candida sphaerica]), HTA1, KLLA0E17413g, HTA2, KLLA0F13332g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Candida sphaerica]), HTB1, KLLA0F13310g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Candida sphaerica])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6uph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uph OCA], [http://pdbe.org/6uph PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uph RCSB], [http://www.ebi.ac.uk/pdbsum/6uph PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uph ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Baker's yeast]]
[[Category: Candida sphaerica]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Arthur, C P]]
[[Category: Arthur, C P]]

Revision as of 21:02, 20 November 2019

Structure of a Yeast Centromeric Nucleosome at 2.7 Angstrom resolutionStructure of a Yeast Centromeric Nucleosome at 2.7 Angstrom resolution

Structural highlights

6uph is a 10 chain structure with sequence from [1], Baker's yeast and Candida sphaerica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:CSE4, CSL2, YKL049C, YKL262 (Baker's yeast), KLLA0_E08647g, KLLA0_E17601g (Candida sphaerica), HTA1, KLLA0E17413g, HTA2, KLLA0F13332g (Candida sphaerica), HTB1, KLLA0F13310g (Candida sphaerica)
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[H2B1_KLULA] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [CENPA_YEAST] Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. Required for functional chromatin architecture at the yeast 2-micron circle partitioning locus and promotes equal plasmid segregation.[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [Q6CMU6_KLULA] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling (By similarity).[SAAS:SAAS00202799]

References

  1. ↑ Stoler S, Keith KC, Curnick KE, Fitzgerald-Hayes M. A mutation in CSE4, an essential gene encoding a novel chromatin-associated protein in yeast, causes chromosome nondisjunction and cell cycle arrest at mitosis. Genes Dev. 1995 Mar 1;9(5):573-86. PMID:7698647
  2. ↑ Meluh PB, Yang P, Glowczewski L, Koshland D, Smith MM. Cse4p is a component of the core centromere of Saccharomyces cerevisiae. Cell. 1998 Sep 4;94(5):607-13. PMID:9741625
  3. ↑ Baker RE, Harris K, Zhang K. Mutations synthetically lethal with cep1 target S. cerevisiae kinetochore components. Genetics. 1998 May;149(1):73-85. PMID:9584087
  4. ↑ Keith KC, Baker RE, Chen Y, Harris K, Stoler S, Fitzgerald-Hayes M. Analysis of primary structural determinants that distinguish the centromere-specific function of histone variant Cse4p from histone H3. Mol Cell Biol. 1999 Sep;19(9):6130-9. PMID:10454560
  5. ↑ Keith KC, Fitzgerald-Hayes M. CSE4 genetically interacts with the Saccharomyces cerevisiae centromere DNA elements CDE I and CDE II but not CDE III. Implications for the path of the centromere dna around a cse4p variant nucleosome. Genetics. 2000 Nov;156(3):973-81. PMID:11063678
  6. ↑ Glowczewski L, Yang P, Kalashnikova T, Santisteban MS, Smith MM. Histone-histone interactions and centromere function. Mol Cell Biol. 2000 Aug;20(15):5700-11. PMID:10891506
  7. ↑ Tanaka T, Cosma MP, Wirth K, Nasmyth K. Identification of cohesin association sites at centromeres and along chromosome arms. Cell. 1999 Sep 17;98(6):847-58. PMID:10499801
  8. ↑ Biggins S, Bhalla N, Chang A, Smith DL, Murray AW. Genes involved in sister chromatid separation and segregation in the budding yeast Saccharomyces cerevisiae. Genetics. 2001 Oct;159(2):453-70. PMID:11606525
  9. ↑ Morey L, Barnes K, Chen Y, Fitzgerald-Hayes M, Baker RE. The histone fold domain of Cse4 is sufficient for CEN targeting and propagation of active centromeres in budding yeast. Eukaryot Cell. 2004 Dec;3(6):1533-43. PMID:15590827 doi:http://dx.doi.org/10.1128/EC.3.6.1533-1543.2004
  10. ↑ Collins KA, Castillo AR, Tatsutani SY, Biggins S. De novo kinetochore assembly requires the centromeric histone H3 variant. Mol Biol Cell. 2005 Dec;16(12):5649-60. Epub 2005 Oct 5. PMID:16207811 doi:http://dx.doi.org/10.1091/mbc.E05-08-0771
  11. ↑ Hajra S, Ghosh SK, Jayaram M. The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-microm circle partitioning locus and promotes equal plasmid segregation. J Cell Biol. 2006 Sep 11;174(6):779-90. PMID:16966420 doi:http://dx.doi.org/jcb.200603042

6uph, resolution 2.70Å

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