6ty8: Difference between revisions
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==In situ structure of BmCPV RNA dependent RNA polymerase at quiescent state== | |||
<StructureSection load='6ty8' size='340' side='right'caption='[[6ty8]], [[Resolution|resolution]] 3.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6ty8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bombyx_mori_cytoplasmic_polyhedrosis_virus Bombyx mori cytoplasmic polyhedrosis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TY8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6TY8 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTA:P1-7-METHYLGUANOSINE-P3-ADENOSINE-5,5-TRIPHOSPHATE'>GTA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ty8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ty8 OCA], [http://pdbe.org/6ty8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ty8 RCSB], [http://www.ebi.ac.uk/pdbsum/6ty8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ty8 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Endogenous RNA transcription characterizes double-stranded RNA (dsRNA) viruses in the Reoviridae, a family that is exemplified by its simple, single-shelled member cytoplasmic polyhedrosis virus (CPV). Because of the lack of in situ structures of the intermediate stages of RNA-dependent RNA polymerase (RdRp) during transcription, it is poorly understood how RdRp detects environmental cues and internal transcriptional states to initiate and coordinate repeated cycles of transcript production inside the capsid. Here, we captured five high-resolution (2.8-3.5 A) RdRp-RNA in situ structures-representing quiescent, initiation, early elongation, elongation and abortive states-under seven experimental conditions of CPV. We observed the 'Y'-form initial RNA fork in the initiation state and the complete transcription bubble in the elongation state. These structures reveal that de novo RNA transcription involves three major conformational changes during state transitions. Our results support an ouroboros model for endogenous conservative transcription in dsRNA viruses. | |||
Conservative transcription in three steps visualized in a double-stranded RNA virus.,Cui Y, Zhang Y, Zhou K, Sun J, Zhou ZH Nat Struct Mol Biol. 2019 Nov;26(11):1023-1034. doi: 10.1038/s41594-019-0320-0., Epub 2019 Nov 6. PMID:31695188<ref>PMID:31695188</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6ty8" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bombyx mori cytoplasmic polyhedrosis virus]] | |||
[[Category: Large Structures]] | |||
[[Category: Cui, Y X]] | |||
[[Category: Sun, J C]] | |||
[[Category: Zhang, Y N]] | |||
[[Category: Zhou, Z H]] | |||
[[Category: Rdrp]] | |||
[[Category: Transferase]] | |||
[[Category: Viral protein]] | |||
Revision as of 18:38, 20 November 2019
In situ structure of BmCPV RNA dependent RNA polymerase at quiescent stateIn situ structure of BmCPV RNA dependent RNA polymerase at quiescent state
Structural highlights
Publication Abstract from PubMedEndogenous RNA transcription characterizes double-stranded RNA (dsRNA) viruses in the Reoviridae, a family that is exemplified by its simple, single-shelled member cytoplasmic polyhedrosis virus (CPV). Because of the lack of in situ structures of the intermediate stages of RNA-dependent RNA polymerase (RdRp) during transcription, it is poorly understood how RdRp detects environmental cues and internal transcriptional states to initiate and coordinate repeated cycles of transcript production inside the capsid. Here, we captured five high-resolution (2.8-3.5 A) RdRp-RNA in situ structures-representing quiescent, initiation, early elongation, elongation and abortive states-under seven experimental conditions of CPV. We observed the 'Y'-form initial RNA fork in the initiation state and the complete transcription bubble in the elongation state. These structures reveal that de novo RNA transcription involves three major conformational changes during state transitions. Our results support an ouroboros model for endogenous conservative transcription in dsRNA viruses. Conservative transcription in three steps visualized in a double-stranded RNA virus.,Cui Y, Zhang Y, Zhou K, Sun J, Zhou ZH Nat Struct Mol Biol. 2019 Nov;26(11):1023-1034. doi: 10.1038/s41594-019-0320-0., Epub 2019 Nov 6. PMID:31695188[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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