Transposase: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
Transposase contains a <scene name='59/596996/Cv/4'>DDE motif in its active site</scene><ref>PMID:18790806</ref> enabling it to coordinate the binding of divalent metal ions. The metal ions affect transposase reactivity. The DDE motif is conserved in transposases and retroviral integrases and is essential for their activity. | Transposase contains a <scene name='59/596996/Cv/4'>DDE motif in its active site</scene><ref>PMID:18790806</ref> enabling it to coordinate the binding of divalent metal ions. The metal ions affect transposase reactivity. The DDE motif is conserved in transposases and retroviral integrases and is essential for their activity. | ||
==3D structures of transposase== | |||
[[Transposase 3D structures]] | |||
</StructureSection> | </StructureSection> | ||
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**[[5unk]] – RED domain – ''Oncorhynchus mykiss'' - NMR<br /> | **[[5unk]] – RED domain – ''Oncorhynchus mykiss'' - NMR<br /> | ||
*DNA-mediated transposase | |||
**[[6pqr]], [[6pqu]], [[6pqx]], [[6pr5]] – DMT – corn earthworm moth – Cryo EM<br /> | |||
*P element transposase | |||
**[[6pe2]], [[6p5a]] – DmPET + DNA – Cryo EM<br /> | |||
**[[3kde]] – DmPET THAP domain + DNA <br /> | |||
*Piggyback transposase | |||
**[[5lme]] – PBT C terminal – cabbage looper - NMR<br /> | |||
}} | }} | ||