6s3f: Difference between revisions
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<StructureSection load='6s3f' size='340' side='right'caption='[[6s3f]], [[Resolution|resolution]] 1.68Å' scene=''> | <StructureSection load='6s3f' size='340' side='right'caption='[[6s3f]], [[Resolution|resolution]] 1.68Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6s3f]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[6s3f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Moringa_oleifera Moringa oleifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6S3F FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.68Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6s3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s3f OCA], [https://pdbe.org/6s3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6s3f RCSB], [https://www.ebi.ac.uk/pdbsum/6s3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6s3f ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/W5S2D2_MOROL W5S2D2_MOROL] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 6s3f" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 6s3f" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Albumin 3D structures|Albumin 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Moringa oleifera]] | [[Category: Moringa oleifera]] | ||
[[Category: Forsyth | [[Category: Forsyth VT]] | ||
[[Category: Haertlein | [[Category: Haertlein M]] | ||
[[Category: Mitchell | [[Category: Mitchell EP]] | ||
[[Category: Mossou | [[Category: Mossou E]] | ||
[[Category: Moulin | [[Category: Moulin M]] | ||
[[Category: Rennie | [[Category: Rennie AR]] | ||
Latest revision as of 08:45, 21 November 2024
Moringa seed protein Mo-CBP3-4Moringa seed protein Mo-CBP3-4
Structural highlights
FunctionPublication Abstract from PubMedSeed extracts from Moringa oleifera are of wide interest for use in water purification where they can play an important role in flocculation; they also have potential as anti-microbial agents. Previous work has focused on the crude protein extract. Here we describe the detailed biophysical characterization of individual proteins from these seeds. The results provide new insights relating to the active compounds involved. One fraction, designated Mo-CBP3, has been characterized at a molecular level using a range of biochemical and biophysical techniques including liquid chromatography, X-ray diffraction, mass spectrometry, and neutron reflection. The interfacial behavior is of particular interest in considering water purification applications and interactions with both charged (e.g. silica) and uncharged (alumina) surfaces were studied. The reflection studies show that, in marked contrast to the crude extract, only a single layer of the purified Mo-CBP3 binds to a silica interface and that there is no binding to an alumina interface. These observations are consistent with the crystallographic structure of Mo-CBP3-4, which is one of the main isoforms of the Mo-CBP3 fraction. The results are put in context of previous studies of the properties of the crude extract. This work shows possible routes to development of separation processes that would be based on the specific properties of individual proteins. Towards a molecular understanding of the water purification properties of Moringa seed proteins.,Moulin M, Mossou E, Signor L, Kieffer-Jaquinod S, Kwaambwa HM, Nermark F, Gutfreund P, Mitchell EP, Haertlein M, Forsyth VT, Rennie AR J Colloid Interface Sci. 2019 Jun 21;554:296-304. doi:, 10.1016/j.jcis.2019.06.071. PMID:31302367[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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