6gp1: Difference between revisions
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<StructureSection load='6gp1' size='340' side='right'caption='[[6gp1]], [[Resolution|resolution]] 1.50Å' scene=''> | <StructureSection load='6gp1' size='340' side='right'caption='[[6gp1]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6gp1]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[6gp1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lobophyllia_hemprichii Lobophyllia hemprichii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GP1 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.504Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7R6:2-[(4E)-4-[(4-hydroxyphenyl)methylidene]-2-[(E)-2-(1H-imidazol-4-yl)ethenyl]-5-oxidanylidene-imidazol-1-yl]ethanoic+acid'>7R6</scene>, <scene name='pdbligand=NFA:PHENYLALANINE+AMIDE'>NFA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gp1 OCA], [https://pdbe.org/6gp1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gp1 RCSB], [https://www.ebi.ac.uk/pdbsum/6gp1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gp1 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q5S6Z9_LOBHE Q5S6Z9_LOBHE] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 6gp1" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 6gp1" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Lobophyllia hemprichii]] | ||
[[Category: Adam | [[Category: Adam V]] | ||
[[Category: Bourgeois | [[Category: Bourgeois D]] | ||
[[Category: Byrdin | [[Category: Byrdin M]] | ||
[[Category: | [[Category: De Zitter E]] | ||
[[Category: | [[Category: Dedecker P]] | ||
[[Category: | [[Category: Van Meervelt L]] | ||
Latest revision as of 13:24, 15 November 2023
Structure of mEos4b in the red long-lived dark stateStructure of mEos4b in the red long-lived dark state
Structural highlights
FunctionPublication Abstract from PubMedGreen-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing interrupted tracks in single-particle-tracking localization microscopy (sptPALM). We identified a long-lived dark state in photoconverted mEos4b that results from isomerization of the chromophore and efficiently absorbs cyan light. Addition of weak 488-nm light swiftly reverts this dark state to the fluorescent state. This strategy largely eliminates slow blinking and enables the recording of longer tracks in sptPALM with minimum effort. Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM.,De Zitter E, Thedie D, Monkemoller V, Hugelier S, Beaudouin J, Adam V, Byrdin M, Van Meervelt L, Dedecker P, Bourgeois D Nat Methods. 2019 Jul 8. pii: 10.1038/s41592-019-0462-3. doi:, 10.1038/s41592-019-0462-3. PMID:31285624[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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