6nef: Difference between revisions

Revision as of 01:50, 7 March 2020

Outer Membrane Cytochrome S Filament from Geobacter SulfurreducensOuter Membrane Cytochrome S Filament from Geobacter Sulfurreducens

6nef, resolution 3.42Å

Structural highlights

6nef is a 1 chain structure with sequence from Geosl. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Experimental data:	Check
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[OMCS_GEOSL] Plays an important role in extracellular electron transfer. Can transfer electrons to insoluble Fe(3+) oxides as well as other extracellular electron acceptors, including Mn(4+) oxide and humic substances (PubMed:16332857, PubMed:20400557, PubMed:21236241). Essential for direct interspecies electron transfer (DIET) in cocultures with G. metallireducens (PubMed:23377933).^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

Electrically conductive pili from Geobacter species, termed bacterial nanowires, are intensely studied for their biological significance and potential in the development of new materials. Using cryo-electron microscopy, we have characterized nanowires from conductive G. sulfurreducens pili preparations that are composed solely of head-to-tail stacked monomers of the six-heme C-type cytochrome OmcS. The unique fold of OmcS - closely wrapped around a continuous stack of hemes that can serve as an uninterrupted path for electron transport - generates a scaffold that supports the unbranched chain of hemes along the central axis of the filament. We present here, at 3.4 A resolution, the structure of this cytochrome-based filament and discuss its possible role in long-range biological electron transport.

Cryo-EM reveals the structural basis of long-range electron transport in a cytochrome-based bacterial nanowire.,Filman DJ, Marino SF, Ward JE, Yang L, Mester Z, Bullitt E, Lovley DR, Strauss M Commun Biol. 2019 Jun 19;2:219. doi: 10.1038/s42003-019-0448-9. eCollection 2019. PMID:31240257^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Mehta T, Coppi MV, Childers SE, Lovley DR. Outer membrane c-type cytochromes required for Fe(III) and Mn(IV) oxide reduction in Geobacter sulfurreducens. Appl Environ Microbiol. 2005 Dec;71(12):8634-41. doi:, 10.1128/AEM.71.12.8634-8641.2005. PMID:16332857 doi:http://dx.doi.org/10.1128/AEM.71.12.8634-8641.2005
↑ Leang C, Qian X, Mester T, Lovley DR. Alignment of the c-type cytochrome OmcS along pili of Geobacter sulfurreducens. Appl Environ Microbiol. 2010 Jun;76(12):4080-4. doi: 10.1128/AEM.00023-10. Epub, 2010 Apr 16. PMID:20400557 doi:http://dx.doi.org/10.1128/AEM.00023-10
↑ Qian X, Mester T, Morgado L, Arakawa T, Sharma ML, Inoue K, Joseph C, Salgueiro CA, Maroney MJ, Lovley DR. Biochemical characterization of purified OmcS, a c-type cytochrome required for insoluble Fe(III) reduction in Geobacter sulfurreducens. Biochim Biophys Acta. 2011 Apr;1807(4):404-12. doi: 10.1016/j.bbabio.2011.01.003., Epub 2011 Jan 12. PMID:21236241 doi:http://dx.doi.org/10.1016/j.bbabio.2011.01.003
↑ Shrestha PM, Rotaru AE, Summers ZM, Shrestha M, Liu F, Lovley DR. Transcriptomic and genetic analysis of direct interspecies electron transfer. Appl Environ Microbiol. 2013 Apr;79(7):2397-404. doi: 10.1128/AEM.03837-12. Epub , 2013 Feb 1. PMID:23377933 doi:http://dx.doi.org/10.1128/AEM.03837-12
↑ Filman DJ, Marino SF, Ward JE, Yang L, Mester Z, Bullitt E, Lovley DR, Strauss M. Cryo-EM reveals the structural basis of long-range electron transport in a cytochrome-based bacterial nanowire. Commun Biol. 2019 Jun 19;2:219. doi: 10.1038/s42003-019-0448-9. eCollection 2019. PMID:31240257 doi:http://dx.doi.org/10.1038/s42003-019-0448-9

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OCA

[1] Mehta T, Coppi MV, Childers SE, Lovley DR. Outer membrane c-type cytochromes required for Fe(III) and Mn(IV) oxide reduction in Geobacter sulfurreducens. Appl Environ Microbiol. 2005 Dec;71(12):8634-41. doi:, 10.1128/AEM.71.12.8634-8641.2005. PMID:16332857 doi:http://dx.doi.org/10.1128/AEM.71.12.8634-8641.2005

[2] Leang C, Qian X, Mester T, Lovley DR. Alignment of the c-type cytochrome OmcS along pili of Geobacter sulfurreducens. Appl Environ Microbiol. 2010 Jun;76(12):4080-4. doi: 10.1128/AEM.00023-10. Epub, 2010 Apr 16. PMID:20400557 doi:http://dx.doi.org/10.1128/AEM.00023-10

[3] Qian X, Mester T, Morgado L, Arakawa T, Sharma ML, Inoue K, Joseph C, Salgueiro CA, Maroney MJ, Lovley DR. Biochemical characterization of purified OmcS, a c-type cytochrome required for insoluble Fe(III) reduction in Geobacter sulfurreducens. Biochim Biophys Acta. 2011 Apr;1807(4):404-12. doi: 10.1016/j.bbabio.2011.01.003., Epub 2011 Jan 12. PMID:21236241 doi:http://dx.doi.org/10.1016/j.bbabio.2011.01.003

[4] Shrestha PM, Rotaru AE, Summers ZM, Shrestha M, Liu F, Lovley DR. Transcriptomic and genetic analysis of direct interspecies electron transfer. Appl Environ Microbiol. 2013 Apr;79(7):2397-404. doi: 10.1128/AEM.03837-12. Epub , 2013 Feb 1. PMID:23377933 doi:http://dx.doi.org/10.1128/AEM.03837-12

[5] Filman DJ, Marino SF, Ward JE, Yang L, Mester Z, Bullitt E, Lovley DR, Strauss M. Cryo-EM reveals the structural basis of long-range electron transport in a cytochrome-based bacterial nanowire. Commun Biol. 2019 Jun 19;2:219. doi: 10.1038/s42003-019-0448-9. eCollection 2019. PMID:31240257 doi:http://dx.doi.org/10.1038/s42003-019-0448-9

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[4]

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@@ Line 1: / Line 1: @@
 ==Outer Membrane Cytochrome S Filament from Geobacter Sulfurreducens==
-<StructureSection load='6nef' size='340' side='right'caption='[[6nef]], [[Resolution|resolution]] 3.42&Aring;' scene=''>
+<SX load='6nef' size='340' side='right' viewer='molstar' caption='[[6nef]], [[Resolution|resolution]] 3.42&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6nef]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacter_sulfurreducens Geobacter sulfurreducens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NEF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6nef]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Geosl Geosl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NEF FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nef OCA], [http://pdbe.org/6nef PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nef RCSB], [http://www.ebi.ac.uk/pdbsum/6nef PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nef ProSAT]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/OMCS_GEOSL OMCS_GEOSL]] Plays an important role in extracellular electron transfer. Can transfer electrons to insoluble Fe(3+) oxides as well as other extracellular electron acceptors, including Mn(4+) oxide and humic substances (PubMed:16332857, PubMed:20400557, PubMed:21236241). Essential for direct interspecies electron transfer (DIET) in cocultures with G. metallireducens (PubMed:23377933).<ref>PMID:16332857</ref> <ref>PMID:20400557</ref> <ref>PMID:21236241</ref> <ref>PMID:23377933</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 16: / Line 18: @@
 </div>
 <div class="pdbe-citations 6nef" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Highest impact structures|Highest impact structures]]
+*[[User:Nikhil Malvankar/Cytochrome nanowires|User:Nikhil Malvankar/Cytochrome nanowires]]
 == References ==
 <references/>
 __TOC__
-</StructureSection>
+</SX>
-[[Category: Geobacter sulfurreducens]]
+[[Category: Geosl]]
 [[Category: Large Structures]]
 [[Category: Bullitt, E]]

6nef: Difference between revisions

Revision as of 01:50, 7 March 2020

Outer Membrane Cytochrome S Filament from Geobacter SulfurreducensOuter Membrane Cytochrome S Filament from Geobacter Sulfurreducens

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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6nef: Difference between revisions

Revision as of 01:50, 7 March 2020

Outer Membrane Cytochrome S Filament from Geobacter SulfurreducensOuter Membrane Cytochrome S Filament from Geobacter Sulfurreducens

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

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