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== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/HTXB_PSEST HTXB_PSEST]] Probably forms part of a binding-protein-dependent hypophosphite transporter. | [[http://www.uniprot.org/uniprot/HTXB_PSEST HTXB_PSEST]] Probably forms part of a binding-protein-dependent hypophosphite transporter. | ||
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== Publication Abstract from PubMed == | |||
Phosphorus acquisition is critical for life. In low phosphate conditions, some species of bacteria have evolved mechanisms to import reduced phosphorus compounds, such as phosphite and hypophosphite, as alternative phosphorus sources. Uptake is facilitated by high-affinity periplasmic binding proteins (PBPs) that bind cargo in the periplasm and shuttle it to an ATP-binding cassette (ABC)-transporter in the bacterial inner membrane. PtxB and HtxB are the PBPs responsible for binding phosphite and hypophosphite, respectively. They recognize the P-H bond of phosphite/hypophosphite via a conserved P-H...pi interaction, which confers nanomolar dissociation constants for their respective ligands. PtxB also has a low-level binding affinity for phosphate and hypophosphite, whilst HtxB can facilitate phosphite uptake in vivo. However, HtxB does not bind phosphate, thus the HtxBCDE transporter has recently been successfully exploited for biocontainment of genetically modified organisms by phosphite-dependent growth. Here we use a combination of X-ray crystallography, NMR and Microscale Thermophoresis to show that phosphite binding to HtxB depends on the protonation state of the ligand, suggesting that pH may effect the efficiency of phosphite uptake by HtxB in biotechnology applications. | |||
Phosphite binding by the HtxB periplasmic binding protein depends on the protonation state of the ligand.,Adams NBP, Robertson AJ, Hunter CN, Hitchcock A, Bisson C Sci Rep. 2019 Jul 15;9(1):10231. doi: 10.1038/s41598-019-46557-2. PMID:31308436<ref>PMID:31308436</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
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== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Latest revision as of 11:33, 24 July 2019
HtxB D206A protein variant from Pseudomonas stutzeri in complex with hypophosphite to 1.12 A resolutionHtxB D206A protein variant from Pseudomonas stutzeri in complex with hypophosphite to 1.12 A resolution
Structural highlights
Function[HTXB_PSEST] Probably forms part of a binding-protein-dependent hypophosphite transporter. Publication Abstract from PubMedPhosphorus acquisition is critical for life. In low phosphate conditions, some species of bacteria have evolved mechanisms to import reduced phosphorus compounds, such as phosphite and hypophosphite, as alternative phosphorus sources. Uptake is facilitated by high-affinity periplasmic binding proteins (PBPs) that bind cargo in the periplasm and shuttle it to an ATP-binding cassette (ABC)-transporter in the bacterial inner membrane. PtxB and HtxB are the PBPs responsible for binding phosphite and hypophosphite, respectively. They recognize the P-H bond of phosphite/hypophosphite via a conserved P-H...pi interaction, which confers nanomolar dissociation constants for their respective ligands. PtxB also has a low-level binding affinity for phosphate and hypophosphite, whilst HtxB can facilitate phosphite uptake in vivo. However, HtxB does not bind phosphate, thus the HtxBCDE transporter has recently been successfully exploited for biocontainment of genetically modified organisms by phosphite-dependent growth. Here we use a combination of X-ray crystallography, NMR and Microscale Thermophoresis to show that phosphite binding to HtxB depends on the protonation state of the ligand, suggesting that pH may effect the efficiency of phosphite uptake by HtxB in biotechnology applications. Phosphite binding by the HtxB periplasmic binding protein depends on the protonation state of the ligand.,Adams NBP, Robertson AJ, Hunter CN, Hitchcock A, Bisson C Sci Rep. 2019 Jul 15;9(1):10231. doi: 10.1038/s41598-019-46557-2. PMID:31308436[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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