4zdm: Difference between revisions
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<StructureSection load='4zdm' size='340' side='right'caption='[[4zdm]], [[Resolution|resolution]] 1.50Å' scene=''> | <StructureSection load='4zdm' size='340' side='right'caption='[[4zdm]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4zdm]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4zdm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pleurobrachia_bachei Pleurobrachia bachei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZDM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZDM FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zdm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zdm OCA], [https://pdbe.org/4zdm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zdm RCSB], [https://www.ebi.ac.uk/pdbsum/4zdm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zdm ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/H6S0J1_PLEBA H6S0J1_PLEBA] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Pleurobrachia bachei]] | ||
[[Category: Grey | [[Category: Grey RJ]] | ||
[[Category: Mayer | [[Category: Mayer ML]] | ||
Revision as of 10:50, 10 May 2023
Pleurobrachia bachei iGluR3 LBD Glycine ComplexPleurobrachia bachei iGluR3 LBD Glycine Complex
Structural highlights
FunctionPublication Abstract from PubMedRecent genome projects for ctenophores have revealed the presence of numerous ionotropic glutamate receptors (iGluRs) in Mnemiopsis leidyi and Pleurobrachia bachei, among our earliest metazoan ancestors. Sequence alignments and phylogenetic analysis show that these form a distinct clade from the well-characterized AMPA, kainate, and NMDA iGluR subtypes found in vertebrates. Although annotated as glutamate and kainate receptors, crystal structures of the ML032222a and PbiGluR3 ligand-binding domains (LBDs) reveal endogenous glycine in the binding pocket, whereas ligand-binding assays show that glycine binds with nanomolar affinity; biochemical assays and structural analysis establish that glutamate is occluded from the binding cavity. Further analysis reveals ctenophore-specific features, such as an interdomain Arg-Glu salt bridge, present only in subunits that bind glycine, but also a conserved disulfide in loop 1 of the LBD that is found in all vertebrate NMDA but not AMPA or kainate receptors. We hypothesize that ctenophore iGluRs are related to an early ancestor of NMDA receptors, suggesting a common evolutionary path for ctenophores and bilaterian species, and suggest that future work should consider both glycine and glutamate as candidate neurotransmitters in ctenophore species. Glycine activated ion channel subunits encoded by ctenophore glutamate receptor genes.,Alberstein R, Grey R, Zimmet A, Simmons DK, Mayer ML Proc Natl Acad Sci U S A. 2015 Nov 3;112(44):E6048-57. doi:, 10.1073/pnas.1513771112. Epub 2015 Oct 12. PMID:26460032[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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