6dl8: Difference between revisions
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<StructureSection load='6dl8' size='340' side='right'caption='[[6dl8]], [[Resolution|resolution]] 3.81Å' scene=''> | <StructureSection load='6dl8' size='340' side='right'caption='[[6dl8]], [[Resolution|resolution]] 3.81Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6dl8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DL8 OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[6dl8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DL8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6DL8 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6dl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dl8 OCA], [http://pdbe.org/6dl8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dl8 RCSB], [http://www.ebi.ac.uk/pdbsum/6dl8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dl8 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The amount of antibody (Ab) variable gene sequence information is expanding rapidly, but our ability to predict the function of Abs from sequence alone is limited. Here, we describe a sequence-to-function prediction method that couples structural data for a single Ab/antigen (Ag) complex with repertoire data. We used a position-specific structure-scoring matrix (P3SM) incorporating structure-prediction scores from Rosetta to identify Ab variable loops that have predicted structural similarity to the influenza virus-specific human Ab CH65. The P3SM approach identified new members of this Ab class. Recombinant Ab expression, crystallography, and virus inhibition assays showed that the HCDR3 loops of the newly identified Abs possessed similar structure and antiviral activity as the comparator CH65. This approach enables discovery of new human Abs with desired structure and function using cDNA repertoires that are obtained readily with current amplicon sequencing techniques. | |||
Identification of Structurally Related Antibodies in Antibody Sequence Databases Using Rosetta-Derived Position-Specific Scoring.,Finn JA, Dong J, Sevy AM, Parrish E, Gilchuk I, Nargi R, Scarlett-Jones M, Reichard W, Bombardi R, Voss TG, Meiler J, Crowe JE Jr Structure. 2020 Oct 6;28(10):1124-1130.e5. doi: 10.1016/j.str.2020.07.012. Epub, 2020 Aug 11. PMID:32783953<ref>PMID:32783953</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6dl8" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Human]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Crowe, J E]] | [[Category: Crowe, J E]] | ||