2j18: Difference between revisions
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[[Image:2j18. | [[Image:2j18.jpg|left|200px]]<br /><applet load="2j18" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="2j18" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="2j18, resolution 1.75Å" /> | caption="2j18, resolution 1.75Å" /> | ||
'''CHLOROPEROXIDASE MIXTURE OF FERRIC AND FERROUS STATES (LOW DOSE DATA SET)'''<br /> | '''CHLOROPEROXIDASE MIXTURE OF FERRIC AND FERROUS STATES (LOW DOSE DATA SET)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2J18 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Leptoxyphium_fumago Leptoxyphium fumago] with NAG, MAN, MN, BR and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] | 2J18 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Leptoxyphium_fumago Leptoxyphium fumago] with NAG, MAN, MN, BR and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] Known structural/functional Site: <scene name='pdbsite=AC1:Br Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J18 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: pyrrolidone carboxylic acid]] | [[Category: pyrrolidone carboxylic acid]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:43:58 2007'' | ||
Revision as of 20:34, 18 December 2007
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CHLOROPEROXIDASE MIXTURE OF FERRIC AND FERROUS STATES (LOW DOSE DATA SET)
OverviewOverview
The X-ray crystallographic analysis of redox-active systems may be, complicated by photoreduction. Although radiolytic reduction by the, probing X-ray beam may be exploited to generate otherwise short-lived, reaction intermediates of metalloproteins, it is generally an undesired, feature. Here, the X-ray-induced reduction of the three heme proteins, myoglobin, cytochrome P450cam and chloroperoxidase has been followed by, on-line UV-Vis absorption spectroscopy. All three systems showed a very, rapid reduction of the heme iron. In chloroperoxidase the change of the, ionization state from ferric to ferrous heme is associated with a movement, of the heme-coordinating water molecule. The influence of the energy of, the incident X-ray photons and of the presence of scavengers on the, apparent reduction rate of ferric myoglobin crystals was analyzed.
About this StructureAbout this Structure
2J18 is a Single protein structure of sequence from Leptoxyphium fumago with NAG, MAN, MN, BR and HEM as ligands. Active as Chloride peroxidase, with EC number 1.11.1.10 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Cryoradiolytic reduction of crystalline heme proteins: analysis by UV-Vis spectroscopy and X-ray crystallography., Beitlich T, Kuhnel K, Schulze-Briese C, Shoeman RL, Schlichting I, J Synchrotron Radiat. 2007 Jan;14(Pt 1):11-23. Epub 2006 Dec 15. PMID:17211068
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