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==Structure of Helix Pomatia agglutinin with zinc and N-acetyl-alpha-D- galactoseamine (GalNAc)==
==Structure of Helix Pomatia agglutinin with zinc and N-acetyl-alpha-D- galactoseamine (GalNAc)==
<StructureSection load='2ccv' size='340' side='right'caption='[[2ccv]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
<StructureSection load='2ccv' size='340' side='right'caption='[[2ccv]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2ccv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Helix_pomatia Helix pomatia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CCV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CCV FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CCV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CCV FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ccv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ccv OCA], [https://pdbe.org/2ccv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ccv RCSB], [https://www.ebi.ac.uk/pdbsum/2ccv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ccv ProSAT]</span></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ce6|2ce6]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ccv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ccv OCA], [http://pdbe.org/2ccv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ccv RCSB], [http://www.ebi.ac.uk/pdbsum/2ccv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ccv ProSAT]</span></td></tr>
</table>
</table>
== Evolutionary Conservation ==
== Evolutionary Conservation ==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ccv ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ccv ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Helix pomatia agglutinin (HPA) is a N-acetylgalactosamine (GalNAc) binding lectin found in the albumen gland of the roman snail. As a constituent of perivitelline fluid, HPA protects fertilized eggs from bacteria and is part of the innate immunity system of the snail. The peptide sequence deduced from gene cloning demonstrates that HPA belongs to a family of carbohydrate-binding proteins recently identified in several invertebrates. This domain is also present in discoidin from the slime mold Dictyostelium discoideum. Investigation of the lectin specificity was performed with the use of glycan arrays, demonstrating that several GalNAc-containing oligosaccharides are bound and rationalizing the use of this lectin as a cancer marker. Titration microcalorimetry performed on the interaction between HPA and GalNAc indicates an affinity in the 10(-4) M range with an enthalpy-driven binding mechanism. The crystal structure of HPA demonstrates the occurrence of a new beta-sandwich lectin fold. The hexameric quaternary state was never observed previously for a lectin. The high resolution structure complex of HPA with GalNAc characterizes a new carbohydrate binding site and rationalizes the observed preference for alphaGalNAc-containing oligosaccharides.
Biochemical and structural analysis of Helix pomatia agglutinin. A hexameric lectin with a novel fold.,Sanchez JF, Lescar J, Chazalet V, Audfray A, Gagnon J, Alvarez R, Breton C, Imberty A, Mitchell EP J Biol Chem. 2006 Jul 21;281(29):20171-80. Epub 2006 May 16. PMID:16704980<ref>PMID:16704980</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2ccv" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Agglutinin 3D structures|Agglutinin 3D structures]]
*[[Agglutinin 3D structures|Agglutinin 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Helix pomatia]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Audfray, A]]
[[Category: Audfray A]]
[[Category: Breton, C]]
[[Category: Breton C]]
[[Category: Chazalet, V]]
[[Category: Chazalet V]]
[[Category: Gagnon, J]]
[[Category: Gagnon J]]
[[Category: Gautier, C]]
[[Category: Gautier C]]
[[Category: Imberty, A]]
[[Category: Imberty A]]
[[Category: Lescar, J]]
[[Category: Lescar J]]
[[Category: Mitchell, E P]]
[[Category: Mitchell EP]]
[[Category: Sanchez, J F]]
[[Category: Sanchez J-F]]
[[Category: Lectin]]
[[Category: Snail]]

Revision as of 18:26, 3 March 2021

Structure of Helix Pomatia agglutinin with zinc and N-acetyl-alpha-D- galactoseamine (GalNAc)Structure of Helix Pomatia agglutinin with zinc and N-acetyl-alpha-D- galactoseamine (GalNAc)

Structural highlights

Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
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