4od6: Difference between revisions
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<StructureSection load='4od6' size='340' side='right'caption='[[4od6]], [[Resolution|resolution]] 1.20Å' scene=''> | <StructureSection load='4od6' size='340' side='right'caption='[[4od6]], [[Resolution|resolution]] 1.20Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4od6]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4od6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans_R1 Deinococcus radiodurans R1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OD6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OD6 FirstGlance]. <br> | ||
</td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4od6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4od6 OCA], [https://pdbe.org/4od6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4od6 RCSB], [https://www.ebi.ac.uk/pdbsum/4od6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4od6 ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/Q9RSZ3_DEIRA Q9RSZ3_DEIRA] Endonuclease that is involved in the suppression of homologous recombination and may therefore have a key role in the control of bacterial genetic diversity (By similarity).[HAMAP-Rule:MF_00092][SAAS:SAAS002625_004_016263] | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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==See Also== | ==See Also== | ||
*[[Endonuclease|Endonuclease]] | *[[Endonuclease 3D structures|Endonuclease 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Deinococcus radiodurans R1]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Hua | [[Category: Hua YJ]] | ||
[[Category: Xu | [[Category: Xu Q]] | ||
[[Category: Zhang | [[Category: Zhang H]] | ||
[[Category: Zhao | [[Category: Zhao Y]] | ||
Revision as of 10:18, 25 January 2023
Structure of Smr domain of MutS2 from Deinococcus radiodurans, Mn2+ soakedStructure of Smr domain of MutS2 from Deinococcus radiodurans, Mn2+ soaked
Structural highlights
FunctionQ9RSZ3_DEIRA Endonuclease that is involved in the suppression of homologous recombination and may therefore have a key role in the control of bacterial genetic diversity (By similarity).[HAMAP-Rule:MF_00092][SAAS:SAAS002625_004_016263] Publication Abstract from PubMedThe MutS2 homologues have been found widespread in most prokaryotes, which are involved in DNA repair and reactive oxygen species detoxification. The C-terminal small mutS-related (Smr) domain is critical for its endonucleolytic activity. However, the detailed catalytic mechanism is still unclear. In this study, we first investigated the in vivo role of drMutS2 in Deinococcus radiodurans, the most radiation-resistant organism exhibits the remarkable DNA repair capacity. mutS2 and recA mutS2 double knockout mutants were constructed because the phenotype was strongly masked by the predominant homologous recombination DNA repair pathway in this bacterium. Compared with the recA mutant, cells devoid of both genes showed increased sensitivity to ionizing radiation and oxidative agents, suggesting that drMutS2 is involved in RecA-independent mechanisms that enhance cellular resistance to oxidative stress-induced DNA damage. Moreover, the basal level of reductase activity and thiamine biosynthesis was induced in the absence of mutS2. To characterize its catalytic residues, the Smr domain was crystallized and soaked in buffer containing manganese ions. In contrast to native crystals, the space group of manganese-derivative crystals transformed from monoclinic to orthorhombic unexpectedly. This type of crystals showed improved diffraction resolution to 1.2A, which has the highest resolution of currently known Smr structures. Structural comparison revealed that three acidic amino-acid residues, which are all located in the alpha1 helix, changed the rotamer states after metal soaking. Mutational analysis of conserved residue glutamic acid 710 to alanine yielded a drMutS2 variant with impaired nuclease activity, and could only partially rescue the radiosensitive phenotype of the mutS2 null strain, indicating that glutamic acid 710 is the catalytic residue. Structural and functional studies of MutS2 from Deinococcus radiodurans.,Zhang H, Xu Q, Lu M, Xu X, Wang Y, Wang L, Zhao Y, Hua Y DNA Repair (Amst). 2014 May 5. pii: S1568-7864(14)00125-6. doi:, 10.1016/j.dnarep.2014.04.012. PMID:24811920[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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