6r2m: Difference between revisions
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==Crystal structure of PssZ from Listeria monocytogenes== | |||
<StructureSection load='6r2m' size='340' side='right'caption='[[6r2m]], [[Resolution|resolution]] 1.62Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6r2m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R2M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R2M FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r2m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r2m OCA], [http://pdbe.org/6r2m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r2m RCSB], [http://www.ebi.ac.uk/pdbsum/6r2m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r2m ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Biofilms are microbial communities that are embedded in the extracellular matrix. The exopolysaccharide (EPS) is a key component of the biofilm matrix that maintains the structure of the biofilm and protects the bacteria from antimicrobials. Microbial glycoside hydrolases have been exploited to disrupt biofilms by breaking down EPSs. PssZ has recently been identified as a glycoside hydrolase that can disperse aggregates of Listeria monocytogenes. In this study, the crystal structure of PssZ has been determined at 1.6 A resolution. PssZ belongs to glycoside hydrolase family 8 and adopts a classical (alpha/alpha)6-barrel fold. This architecture forms a deep groove which may serve as the substrate-binding pocket. The conserved catalytic residues (Glu72, Trp110, Asn119, Phe167, Tyr183 and Asp232) are localized at the centre of the groove. This crystal structure will help to improve the understanding of the hydrolytic mechanism of PssZ and its application as a biofilm disrupter. | |||
Crystal structure of the glycoside hydrolase PssZ from Listeria monocytogenes.,Wu H, Qiao S, Li D, Guo L, Zhu M, Ma LZ Acta Crystallogr F Struct Biol Commun. 2019 Jul 1;75(Pt 7):501-506. doi:, 10.1107/S2053230X19008100. Epub 2019 Jun 28. PMID:31282870<ref>PMID:31282870</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6r2m" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Cheng, J]] | |||
[[Category: Li, D]] | [[Category: Li, D]] | ||
[[Category: | [[Category: Ma, L]] | ||
[[Category: Qiao, S]] | [[Category: Qiao, S]] | ||
[[Category: Wu, H]] | [[Category: Wu, H]] | ||
[[Category: Exopolysacharide]] | |||
[[Category: Extracellular]] | |||
[[Category: Glycosylhydrolase]] | |||
[[Category: Hydrolase]] | |||
[[Category: Monomer]] | |||
Latest revision as of 09:28, 24 July 2019
Crystal structure of PssZ from Listeria monocytogenesCrystal structure of PssZ from Listeria monocytogenes
Structural highlights
Publication Abstract from PubMedBiofilms are microbial communities that are embedded in the extracellular matrix. The exopolysaccharide (EPS) is a key component of the biofilm matrix that maintains the structure of the biofilm and protects the bacteria from antimicrobials. Microbial glycoside hydrolases have been exploited to disrupt biofilms by breaking down EPSs. PssZ has recently been identified as a glycoside hydrolase that can disperse aggregates of Listeria monocytogenes. In this study, the crystal structure of PssZ has been determined at 1.6 A resolution. PssZ belongs to glycoside hydrolase family 8 and adopts a classical (alpha/alpha)6-barrel fold. This architecture forms a deep groove which may serve as the substrate-binding pocket. The conserved catalytic residues (Glu72, Trp110, Asn119, Phe167, Tyr183 and Asp232) are localized at the centre of the groove. This crystal structure will help to improve the understanding of the hydrolytic mechanism of PssZ and its application as a biofilm disrupter. Crystal structure of the glycoside hydrolase PssZ from Listeria monocytogenes.,Wu H, Qiao S, Li D, Guo L, Zhu M, Ma LZ Acta Crystallogr F Struct Biol Commun. 2019 Jul 1;75(Pt 7):501-506. doi:, 10.1107/S2053230X19008100. Epub 2019 Jun 28. PMID:31282870[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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