|
|
| Line 3: |
Line 3: |
| <StructureSection load='4c20' size='340' side='right'caption='[[4c20]], [[Resolution|resolution]] 2.41Å' scene=''> | | <StructureSection load='4c20' size='340' side='right'caption='[[4c20]], [[Resolution|resolution]] 2.41Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4c20]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strpn Strpn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C20 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C20 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[4c20]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C20 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C20 FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4c21|4c21]], [[4c22|4c22]], [[4c23|4c23]], [[4c24|4c24]], [[4c25|4c25]]</td></tr>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c20 OCA], [https://pdbe.org/4c20 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c20 RCSB], [https://www.ebi.ac.uk/pdbsum/4c20 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c20 ProSAT]</span></td></tr> |
| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-fucose_isomerase L-fucose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.25 5.3.1.25] </span></td></tr>
| |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c20 OCA], [http://pdbe.org/4c20 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c20 RCSB], [http://www.ebi.ac.uk/pdbsum/4c20 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c20 ProSAT]</span></td></tr> | |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/FUCI_STRPN FUCI_STRPN]] Converts the aldose L-fucose into the corresponding ketose L-fuculose (By similarity). | | [[https://www.uniprot.org/uniprot/FUCI_STRPN FUCI_STRPN]] Converts the aldose L-fucose into the corresponding ketose L-fuculose (By similarity). |
| <div style="background-color:#fffaf0;">
| |
| == Publication Abstract from PubMed ==
| |
| Fucose metabolism pathways are present in many bacterial species and typically contain the central fucose-processing enzymes fucose isomerase (FcsI), fuculose kinase (FcsK), and fuculose-1-phosphate aldolase (FcsA). Fucose initially undergoes isomerization by FcsI producing fuculose, which is then phosphorylated by FcsK. FcsA cleaves the fuculose-1-phosphate product into lactaldehyde and dihydroxyacetone phosphate, which can be incorporated into central metabolism allowing the bacterium to use fucose as an energy source. Streptococcus pneumoniae has fucose-processing operons containing homologs of FcsI, FcsK, and FcsA; however, this bacterium appears unable to utilize fucose as an energy source. To investigate this contradiction, we performed biochemical and structural studies of the S. pneumoniae fucose-processing enzymes SpFcsI, SpFcsK, and SpFcsA. These enzymes are demonstrated to act in a sequential manner to ultimately produce dihydroxyacetone phosphate and have structural features entirely consistent with their observed biochemical activities. Analogous to the regulation of the Escherichia coli fucose utilization operon, fuculose-1-phosphate appears to act as an inducing molecule for activation of the S. pneumoniae fucose operon. Despite our evidence that S. pneumoniae appears to have the appropriate regulatory and biochemical machinery for fucose metabolism, we confirmed the inability of the S. pneumoniae TIGR4 strain to grow on fucose or on the H-disaccharide, which is the probable substrate of the transporter for the pathway. On the basis of these observations, we postulate that the S. pneumoniae fucose-processing pathway has a non-metabolic role in the interaction of this bacterium with its human host.
| |
| | |
| Structural and Functional Analysis of Fucose-Processing Enzymes from Streptococcus pneumoniae.,Higgins MA, Suits MD, Marsters C, Boraston AB J Mol Biol. 2013 Dec 12. pii: S0022-2836(13)00748-1. doi:, 10.1016/j.jmb.2013.12.006. PMID:24333485<ref>PMID:24333485</ref>
| |
| | |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| |
| </div>
| |
| <div class="pdbe-citations 4c20" style="background-color:#fffaf0;"></div>
| |
| == References ==
| |
| <references/>
| |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: L-fucose isomerase]]
| |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Strpn]] | | [[Category: Streptococcus pneumoniae TIGR4]] |
| [[Category: Boraston, A B]] | | [[Category: Boraston AB]] |
| [[Category: Higgins, M A]] | | [[Category: Higgins MA]] |
| [[Category: Marsters, C]] | | [[Category: Marsters C]] |
| [[Category: Suits, M D.L]] | | [[Category: Suits MDL]] |
| [[Category: Fucose processing]]
| |
| [[Category: Isomerase]]
| |