2cfp: Difference between revisions
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[[Image:2cfp.gif|left|200px]]<br /> | [[Image:2cfp.gif|left|200px]]<br /><applet load="2cfp" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="2cfp" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="2cfp, resolution 3.30Å" /> | caption="2cfp, resolution 3.30Å" /> | ||
'''SUGAR FREE LACTOSE PERMEASE AT ACIDIC PH'''<br /> | '''SUGAR FREE LACTOSE PERMEASE AT ACIDIC PH'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2CFP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with HG as [http://en.wikipedia.org/wiki/ligand ligand]. | 2CFP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with HG as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC2:Hg Binding Site For Chain A'>AC2</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CFP OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transport mechanism]] | [[Category: transport mechanism]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:20:55 2007'' | ||
Revision as of 20:11, 18 December 2007
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SUGAR FREE LACTOSE PERMEASE AT ACIDIC PH
OverviewOverview
Cation-coupled active transport is an essential cellular process found, ubiquitously in all living organisms. Here, we present two novel, ligand-free X-ray structures of the lactose permease (LacY) of Escherichia, coli determined at acidic and neutral pH, and propose a model for the, mechanism of coupling between lactose and H+ translocation. No, sugar-binding site is observed in the absence of ligand, and deprotonation, of the key residue Glu269 is associated with ligand binding. Thus, substrate induces formation of the sugar-binding site, as well as the, initial step in H+ transduction.
About this StructureAbout this Structure
2CFP is a Single protein structure of sequence from Escherichia coli with HG as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY., Mirza O, Guan L, Verner G, Iwata S, Kaback HR, EMBO J. 2006 Mar 22;25(6):1177-83. Epub 2006 Mar 9. PMID:16525509
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