Boris Brumshtein
Joined 28 October 2007
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=CV= | |||
[[Image:UnderConstruction.gif]] | ==PERSONAL INFORMATION== | ||
Name: Boris Brumshtein | |||
Languages: English, Hebrew and Russian | |||
Current residence: Rehovot, Israel. | |||
==CONTACT INFORMATION== | |||
Department of Structural Biology, Weizmann Institute of Science | |||
Rehovot, 76100, Israel | |||
E-mail: Boris {dot} Brumshtein {at} weizmann {dot} ac {dot} il | |||
Tell: +972-52-6902631 (private) | |||
+972-8-9342647 (work) | |||
==EDUCATION== | |||
2003 - present Direct PhD program at the Weizmann Institute of Science, Faculty of Chemistry, Department of Structural Biology. | |||
Estimated graduation - summer 2008. Scientific advisors: Prof. Joel L. Sussman, Prof. Israel Silman, Prof. Anthony H. Futerman | |||
Thesis topic: "Structural and biochemical studies on acid-beta-glucosidase, the defected protein in Gaucher disease". | |||
2002-2003 MSc student, Weizmann Institute of Science, Faculty of Chemistry, Department of Structural Biology. | |||
Scientific advisor: Prof. Joel Sussman. | |||
1999-2002 BA in Biochemistry, Technion, Israel Institute of Science and Technology, Faculty of Chemistry, cum laude. | |||
==QUALIFICATIONS== | |||
In my research I employ different protein biochemistry, enzymology and crystallography methods, thus I gained a lot of experience in the following techniques: | |||
====Crystallography==== | |||
- Protein crystallization utilizing microbatch crystallization, IMPAX I-5, Oryx and Mosquito crystallization robots. | |||
- ''Ab initio'' crystallization and crystals optimization. Various crystallization techniques, such as: soaking, co-crystallization, crystallization in presence of heavy metals for phasing, etc. | |||
- Crystal handling and manipulations, micro-manipulations with ZEISS microscope/manipulator system. | |||
- Data acquisition using Rigaku Raxis 4/4++ or synchrotron x-ray sources. | |||
- X-ray data processing software: XDS, HKL2000. | |||
- Protein structure determination using MR, SIR, MIR, MAD, SIRAS approaches with CNS, SHELX and CCP4 software suits. | |||
- Molecular modeling, visualization and analysis software: PyMol, Coot, XtalView, O, CCP4, APBS. | |||
- Docking and small molecule/drug database mining with AutoDock - drug docking and virtual screening, Cambridge Structure Database (CSB), ZINC, ChemOffice. | |||
====Molecular biology and biochemistry==== | |||
- Protein cloning and expression in E.Coli. | |||
- Biochemical and protein purification techniques: HPLC, FPLC, Ni-columns, Ion | |||
exchange columns, acryl-amide gels, size exclusion, affinity chromatography, | |||
gradient and analytical ultracentrifugation, ultra-filtration, Mini Prep cell, etc. | |||
- Drug-Protein interaction analysis employing various structural, biochemical and | |||
biophysical assays such as: enzymatic assays, ELIZA, circular dichroism, dynamic | |||
light scattering, mass-spec, BiaCore, analytical ultracentrifugation, etc. | |||
====Programming languages==== | |||
-Unix, C, Perl, Matlab, Java (basic programming). | |||
The page is always under construction ; last update 8-Jun-2008 | |||
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