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==Cryo-EM Structure of an Extracellular Contractile Injection System, PVC sheath complex in contracted state== | |||
<StructureSection load='6j0c' size='340' side='right'caption='[[6j0c]], [[Resolution|resolution]] 3.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6j0c]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J0C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J0C FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j0c OCA], [http://pdbe.org/6j0c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j0c RCSB], [http://www.ebi.ac.uk/pdbsum/6j0c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j0c ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Contractile injection systems (CISs) are cell-puncturing nanodevices that share ancestry with contractile tail bacteriophages. Photorhabdus virulence cassette (PVC) represents one group of extracellular CISs that are present in both bacteria and archaea. Here, we report the cryo-EM structure of an intact PVC from P. asymbiotica. This over 10-MDa device resembles a simplified T4 phage tail, containing a hexagonal baseplate complex with six fibers and a capped 117-nanometer sheath-tube trunk. One distinct feature of the PVC is the presence of three variants for both tube and sheath proteins, indicating a functional specialization of them during evolution. The terminal hexameric cap docks onto the topmost layer of the inner tube and locks the outer sheath in pre-contraction state with six stretching arms. Our results on the PVC provide a framework for understanding the general mechanism of widespread CISs and pave the way for using them as delivery tools in biological or therapeutic applications. | |||
Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System.,Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019, Mar 21. PMID:30905475<ref>PMID:30905475</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6j0c" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Cai, B]] | |||
[[Category: Cheng, J]] | |||
[[Category: Gao, N]] | |||
[[Category: Huang, Y]] | |||
[[Category: Jiang, F]] | |||
[[Category: Jin, Q]] | |||
[[Category: Li, N]] | |||
[[Category: Wang, X]] | |||
[[Category: Wang, Y]] | |||
[[Category: Yang, J]] | |||
[[Category: Yang, Y]] | |||
[[Category: Assembly]] | |||
[[Category: Bacteriophage-like]] | |||
[[Category: Contractile injection system]] | |||
[[Category: Photorhabdus asymbiotica]] | |||
[[Category: Protein transport]] | |||
[[Category: Pvc]] | |||
Revision as of 10:24, 10 April 2019
Cryo-EM Structure of an Extracellular Contractile Injection System, PVC sheath complex in contracted stateCryo-EM Structure of an Extracellular Contractile Injection System, PVC sheath complex in contracted state
Structural highlights
Publication Abstract from PubMedContractile injection systems (CISs) are cell-puncturing nanodevices that share ancestry with contractile tail bacteriophages. Photorhabdus virulence cassette (PVC) represents one group of extracellular CISs that are present in both bacteria and archaea. Here, we report the cryo-EM structure of an intact PVC from P. asymbiotica. This over 10-MDa device resembles a simplified T4 phage tail, containing a hexagonal baseplate complex with six fibers and a capped 117-nanometer sheath-tube trunk. One distinct feature of the PVC is the presence of three variants for both tube and sheath proteins, indicating a functional specialization of them during evolution. The terminal hexameric cap docks onto the topmost layer of the inner tube and locks the outer sheath in pre-contraction state with six stretching arms. Our results on the PVC provide a framework for understanding the general mechanism of widespread CISs and pave the way for using them as delivery tools in biological or therapeutic applications. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System.,Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019, Mar 21. PMID:30905475[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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