3n0r: Difference between revisions

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==Structure of the PhyR stress response regulator at 1.25 Angstrom resolution==
==Structure of the PhyR stress response regulator at 1.25 Angstrom resolution==
<StructureSection load='3n0r' size='340' side='right' caption='[[3n0r]], [[Resolution|resolution]] 1.25&Aring;' scene=''>
<StructureSection load='3n0r' size='340' side='right'caption='[[3n0r]], [[Resolution|resolution]] 1.25&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3n0r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cauvi Cauvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N0R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N0R FirstGlance]. <br>
<table><tr><td colspan='2'>[[3n0r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caulobacter_vibrioides Caulobacter vibrioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N0R FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.251&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CC_3477, PhyR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=155892 CAUVI])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n0r OCA], [https://pdbe.org/3n0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n0r RCSB], [https://www.ebi.ac.uk/pdbsum/3n0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n0r ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n0r OCA], [http://pdbe.org/3n0r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3n0r RCSB], [http://www.ebi.ac.uk/pdbsum/3n0r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3n0r ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9A2S9_CAUVC Q9A2S9_CAUVC]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3n0r ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3n0r ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
PhyR is a hybrid stress regulator conserved in alpha-proteobacteria that contains an N-terminal sigma-like (SL) domain and a C-terminal receiver domain. Phosphorylation of the receiver domain is known to promote binding of the SL domain to an anti-sigma factor. PhyR thus functions as an anti-anti-sigma factor in its phosphorylated state. We present genetic evidence that Caulobacter crescentus PhyR is a phosphorylation-dependent stress regulator that functions in the same pathway as sigma(T) and its anti-sigma factor, NepR. Additionally, we report the X-ray crystal structure of PhyR at 1.25 A resolution, which provides insight into the mechanism of anti-anti-sigma regulation. Direct intramolecular contact between the PhyR receiver and SL domains spans regions sigma and sigma, likely serving to stabilize the SL domain in a closed conformation. The molecular surface of the receiver domain contacting the SL domain is the structural equivalent of alpha4-beta5-alpha5, which is known to undergo dynamic conformational change upon phosphorylation in a diverse range of receiver proteins. We propose a structural model of PhyR regulation in which receiver phosphorylation destabilizes the intramolecular interaction between SL and receiver domains, thereby permitting regions sigma and sigma in the SL domain to open about a flexible connector loop and bind anti-sigma factor.
A structural model of anti-anti-sigma inhibition by a two-component receiver domain: the PhyR stress response regulator.,Herrou J, Foreman R, Fiebig A, Crosson S Mol Microbiol. 2010 Oct;78(2):290-304. doi:, 10.1111/j.1365-2958.2010.07323.x. Epub 2010 Aug 18. PMID:20735776<ref>PMID:20735776</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3n0r" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Response regulator|Response regulator]]
*[[Response regulator 3D structure|Response regulator 3D structure]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Cauvi]]
[[Category: Caulobacter vibrioides]]
[[Category: Crosson, S]]
[[Category: Large Structures]]
[[Category: Herrou, J]]
[[Category: Crosson S]]
[[Category: Receiver]]
[[Category: Herrou J]]
[[Category: Response regulator]]
[[Category: Sigma factor]]
[[Category: Signaling protein]]
[[Category: Two-component signal transduction]]

Revision as of 09:41, 3 April 2024

Structure of the PhyR stress response regulator at 1.25 Angstrom resolutionStructure of the PhyR stress response regulator at 1.25 Angstrom resolution

Structural highlights

3n0r is a 1 chain structure with sequence from Caulobacter vibrioides. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.251Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9A2S9_CAUVC

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

3n0r, resolution 1.25Å

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OCA
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