6cel: Difference between revisions

Revision as of 22:39, 4 May 2008

CBH1 (E212Q) CELLOPENTAOSE COMPLEX

OverviewOverview

Detailed information has been obtained, by means of protein X-ray crystallography, on how a cellulose chain is bound in the cellulose-binding tunnel of cellobiohydrolase I (CBHI), the major cellulase in the hydrolysis of native, crystalline cellulose by the fungus Trichoderma reesei. Three high-resolution crystal structures of different catalytically deficient mutants of CBHI in complex with cellotetraose, cellopentaose and cellohexaose have been refined at 1.9, 1.7 and 1.9 A resolution, respectively. The observed binding of cellooligomers in the tunnel allowed unambiguous identification of ten well-defined subsites for glucosyl units that span a length of approximately 50 A. All bound oligomers have the same directionality and orientation, and the positions of the glucosyl units in each binding site agree remarkably well between the different complexes. The binding mode observed here corresponds to that expected during productive binding of a cellulose chain. The structures support the hypothesis that hydrolysis by CBHI proceeds from the reducing towards the non-reducing end of a cellulose chain, and they provide a structural explanation for the observed distribution of initial hydrolysis products.

About this StructureAbout this Structure

6CEL is a Single protein structure of sequence from Hypocrea jecorina. Full crystallographic information is available from OCA.

ReferenceReference

High-resolution crystal structures reveal how a cellulose chain is bound in the 50 A long tunnel of cellobiohydrolase I from Trichoderma reesei., Divne C, Stahlberg J, Teeri TT, Jones TA, J Mol Biol. 1998 Jan 16;275(2):309-25. PMID:9466911 Page seeded by OCA on Sun May 4 22:39:34 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:6cel.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 6cel |SIZE=350|CAPTION= <scene name='initialview01'>6cel</scene>, resolution 1.7&Aring;
+The line below this paragraph, containing "STRUCTURE_6cel", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=CAT:Catalytic+Site+Including+Mutation+E212q'>CAT</scene> and <scene name='pdbsite=COB:Co-Binding+Site+On+Crystallographic+Dyad.+The+Metal+Ion+...'>COB</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase Cellulose 1,4-beta-cellobiosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= CBH1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=51453 Hypocrea jecorina])
+-->
-|DOMAIN=
+{{STRUCTURE_6cel|  PDB=6cel  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cel OCA], [http://www.ebi.ac.uk/pdbsum/6cel PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=6cel RCSB]</span>
-}}
 '''CBH1 (E212Q) CELLOPENTAOSE COMPLEX'''
@@ Line 29: / Line 26: @@
 [[Category: Jones, T A.]]
 [[Category: Stahlberg, J.]]
-[[Category: cellulose degradation]]
+[[Category: Cellulose degradation]]
-[[Category: glycoprotein]]
+[[Category: Glycoprotein]]
-[[Category: glycosidase]]
+[[Category: Glycosidase]]
-[[Category: glycosylated protein]]
+[[Category: Glycosylated protein]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 22:39:34 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:42:46 2008''