6cgt: Difference between revisions

Revision as of 22:39, 4 May 2008

HOXA COMPLEX OF CYCLODEXTRIN GLYCOSYLTRANSFERASE MUTANT

OverviewOverview

Bacterial cyclodextrin glycosyltransferases use starch to produce cyclic maltooligosaccharides (cyclodextrins) which are of interest in various applications. The cyclization reaction gives rise to a spectrum of ring sizes consisting of predominantly six to eight glucosyl units. Using the enzyme from Bacillus circulans strain no. 8, binding studies have been performed with several substrates and analogues. The observed binding modes differ in detail, but agree in general with data on homologous enzymes. Based on these binding studies, two mutations were designed that changed the production spectrum from the predominant product beta-cyclodextrin of the wild-type enzyme towards gamma-cyclodextrin, which is of practical interest because it is rare and can encapsulate larger nonpolar compounds.

About this StructureAbout this Structure

6CGT is a Single protein structure of sequence from Bacillus circulans. Full crystallographic information is available from OCA.

ReferenceReference

Substrate binding to a cyclodextrin glycosyltransferase and mutations increasing the gamma-cyclodextrin production., Parsiegla G, Schmidt AK, Schulz GE, Eur J Biochem. 1998 Aug 1;255(3):710-7. PMID:9738912 Page seeded by OCA on Sun May 4 22:39:34 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:6cgt.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 6cgt |SIZE=350|CAPTION= <scene name='initialview01'>6cgt</scene>, resolution 2.6&Aring;
+The line below this paragraph, containing "STRUCTURE_6cgt", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DAG:4,6-DIDEOXY-4-AMINO-BETA-D-GLUCOPYRANOSIDE'>DAG</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=OPG:OXIRANPSEUDOGLUCOSE'>OPG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_6cgt|  PDB=6cgt  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cgt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cgt OCA], [http://www.ebi.ac.uk/pdbsum/6cgt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=6cgt RCSB]</span>
-}}
 '''HOXA COMPLEX OF CYCLODEXTRIN GLYCOSYLTRANSFERASE MUTANT'''
@@ Line 28: / Line 25: @@
 [[Category: Parsiegla, G.]]
 [[Category: Schulz, G E.]]
-[[Category: cyclodextrin]]
+[[Category: Cyclodextrin]]
-[[Category: glycosyltransferase]]
+[[Category: Glycosyltransferase]]
-[[Category: starch degradation]]
+[[Category: Starch degradation]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 22:39:34 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:42:44 2008''