Sandbox1503: Difference between revisions

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'''Secondary structure:'''
'''Secondary structure:'''
[[Image:secondary structure.jpg]]
 


== Structural highlights ==
== Structural highlights ==

Revision as of 21:42, 11 January 2019

3vuf Protein(title here)3vuf Protein(title here)

3vuf is a 1 chain structure protein which is synthesized by the Japanese rice. it’s an enzyme, more accurately a transferase. It’s a NDP-glucose--starch glucosyltransferase, which is involved in the synthesis of amylose in the rice. we found this enzyme in chloroplast and in amyloplast.

Function

[SSG1_ORYSJ] Required for the synthesis of amylose in endosperm.[HAMAP-Rule:MF_00484]

Publication Abstract from PubMed

The catalytic domain of rice (Oryza sativa japonica) granule bound starch synthase I (OsGBSSI-CD) was overexpressed and the three-dimensional structures of the ligand-free and ADP-bound forms were determined. The structures were similar to those reported for bacterial and archaeal glycogen synthases, which belong to glycosyltransferase family 5. They had Rossmann fold N- and C-domains connected by canonical two-hinge peptides, and an interdomain disulfide bond that appears to be conserved in the Poaceae plant family. The presence of three covalent linkages might explain why both OsGBSSI-CD structures adopted only the closed domain arrangement.

Interdomain Disulfide Bridge in the Rice Granule Bound Starch Synthase I Catalytic Domain as Elucidated by X-Ray Structure Analysis.,Momma M, Fujimoto Z Biosci Biotechnol Biochem. 2012 Aug 7. PMID:22878205[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Disease

The protein 3vuf has allergenic properties, for example in mammals it can bind to Immunoglobulin E (IgE) causing an allergic response.

Relevance

Structure

Primary structure (amino acid sequence):


MSALTTSQLA TSATGFGIAD RSAPSSLLRH GFQGLKPRSP AGGDATSLSV

TTSARATPKQ QRSVQRGSRR FPSVVVYATG AGMNVVFVGA EMAPWSKTGG

LGDVLGGLPP AMAANGHRVM VISPRYDQYK DAWDTSVVAE IKVADRYERV

RFFHCYKRGV DRVFIDHPSF LEKVWGKTGE KIYGPDTGVD YKDNQMRFSL

LCQAALEAPR ILNLNNNPYF KGTYGEDVVF VCNDWHTGPL ASYLKNNYQP

NGIYRNAKVA FCIHNISYQG RFAFEDYPEL NLSERFRSSF DFIDGYDTPV

EGRKINWMKA GILEADRVLT VSPYYAEELI SGIARGCELD NIMRLTGITG

IVNGMDVSEW DPSKDKYITA KYDATTAIEA KALNKEALQA EAGLPVDRKI

PLIAFIGRLE EQKGPDVMAA AIPELMQEDV QIVLLGTGKK KFEKLLKSME

EKYPGKVRAV VKFNAPLAHL IMAGADVLAV PSRFEPCGLI QLQGMRYGTP

CACASTGGLV DTVIEGKTGF HMGRLSVDCK VVEPSDVKKV AATLKRAIKV

VGTPAYEEMV RNCMNQDLSW KGPAKNWENV LLGLGVAGSA PGIEGDEIAP

LAKENVAAP


Secondary structure:


Structural highlights

3vuf is a 1 chain structure with sequence from Japanese rice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:,
Gene:WAXY, WX, WX-B, Os06g0133000, LOC_Os06g04200, 134P10.7, P0679C08.19 (Japanese rice)
Activity:NDP-glucose--starch glucosyltransferase, with EC number 2.4.1.242
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

This is a sample scene created with SAT to by Group, and another to make of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.


3vuf, resolution 3.00Å

Drag the structure with the mouse to rotate

ReferencesReferences

  1. Momma M, Fujimoto Z. Interdomain Disulfide Bridge in the Rice Granule Bound Starch Synthase I Catalytic Domain as Elucidated by X-Ray Structure Analysis. Biosci Biotechnol Biochem. 2012 Aug 7. PMID:22878205

</StructureSection>

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Loïc Plessis