Sandbox Reserved 1489: Difference between revisions
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===Structure of the substrate binding site=== | ===Structure of the substrate binding site=== | ||
A large cavity on the inward facing side of the protein is made up of the neighboring surfaces of TMs 1, 3, 5, 6 and 8. This cavity connects the substrate and cation binding sites. | |||
The substrate binding site is located at the break of the TMs 1 and 6. (''Figure 2'') | The substrate binding site is located at the break of the TMs 1 and 6. (''Figure 2'') | ||
The substrate, the benzyl-hydantoin interacts with the amino acids of the binding site. The hydantoin group establisches pi-stacking interactions with the indole ring of Trp 117 and Trp 220 and hydrogen bonds with Asn 318 and Gln 121. The benzyl ring interacts with Trp 220 and Gln 42 (''Figure 3''). | The substrate, the benzyl-hydantoin interacts with the amino acids of the binding site. The hydantoin group establisches pi-stacking interactions with the indole ring of Trp 117 on TM3 and Trp 220 on TM6 and hydrogen bonds with Asn 318 and Gln 121. The benzyl ring interacts with Trp 220 and Gln 42 (''Figure 3''). | ||
[[Image:Substrate binding site-.jpg]] | [[Image:Substrate binding site-.jpg]] | ||