3l7l: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Structure of the Wall Teichoic Acid Polymerase TagF, H444N + CDPG (30 minute soak)== | ==Structure of the Wall Teichoic Acid Polymerase TagF, H444N + CDPG (30 minute soak)== | ||
<StructureSection load='3l7l' size='340' side='right' caption='[[3l7l]], [[Resolution|resolution]] 2.95Å' scene=''> | <StructureSection load='3l7l' size='340' side='right'caption='[[3l7l]], [[Resolution|resolution]] 2.95Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3l7l]] is a 4 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3l7l]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staeq Staeq]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L7L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L7L FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C2G:[CYTIDINE-5-PHOSPHATE]+GLYCERYLPHOSPHORIC+ACID+ESTER'>C2G</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C2G:[CYTIDINE-5-PHOSPHATE]+GLYCERYLPHOSPHORIC+ACID+ESTER'>C2G</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3l7i|3l7i]], [[3l7j|3l7j]], [[3l7k|3l7k]], [[3l7m|3l7m]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3l7i|3l7i]], [[3l7j|3l7j]], [[3l7k|3l7k]], [[3l7m|3l7m]]</div></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SERP1960, tagF ([ | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SERP1960, tagF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=176279 STAEQ])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l7l OCA], [https://pdbe.org/3l7l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l7l RCSB], [https://www.ebi.ac.uk/pdbsum/3l7l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l7l ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
| Line 32: | Line 32: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Staeq]] | [[Category: Staeq]] | ||
[[Category: Lovering, A L]] | [[Category: Lovering, A L]] | ||
Revision as of 15:31, 13 October 2021
Structure of the Wall Teichoic Acid Polymerase TagF, H444N + CDPG (30 minute soak)Structure of the Wall Teichoic Acid Polymerase TagF, H444N + CDPG (30 minute soak)
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedTeichoic acid polymers are composed of polyol-phosphate units and form a major component of Gram-positive bacterial cell walls. These anionic compounds perform a multitude of important roles in bacteria and are synthesized by monotopic membrane proteins of the TagF polymerase family. We have determined the structure of Staphylococcus epidermidis TagF to 2.7-A resolution from a construct that includes both the membrane-targeting region and the glycerol-phosphate polymerase domains. TagF possesses a helical region for interaction with the lipid bilayer, placing the active site at a suitable distance for access to the membrane-bound substrate. Characterization of active-site residue variants and analysis of a CDP-glycerol substrate complex suggest a mechanism for polymer synthesis. With the importance of teichoic acid in Gram-positive physiology, this elucidation of the molecular details of TagF function provides a critical new target in the development of novel anti-infectives. Structure of the bacterial teichoic acid polymerase TagF provides insights into membrane association and catalysis.,Lovering AL, Lin LY, Sewell EW, Spreter T, Brown ED, Strynadka NC Nat Struct Mol Biol. 2010 May;17(5):582-9. Epub 2010 Apr 18. PMID:20400947[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||||||