3nuc: Difference between revisions

Revision as of 22:08, 4 May 2008

STAPHLOCOCCAL NUCLEASE, 1-N-PROPANE THIOL DISULFIDE TO V23C VARIANT

OverviewOverview

The structures of several variants of staphylococcal nuclease with long flexible unnatural amino acid side chains in the hydrophobic core have been determined by X-ray crystallography. The unnatural amino acids are disulfide moieties between the lone cysteine residue in V23C nuclease and methane, ethane, 1-n-propane, 1-n-butane, 1-n-pentane, and 2-hydroxyethyl thiols. We have examined changes in the core packing of these mutants. Side chains as large as the 1-n-propyl cysteine disulfide can be incorporated without perturbation of the structure. This is due, in part, to cavities present in the wild-type protein. The longest side chains are not well defined, even though they remain buried within the protein interior. These results suggest that the enthalpy-entropy balance that governs the rigidity of protein interiors favors tight packing only weakly. Additionally, the tight packing observed normally in protein interiors may reflect, in part, the limited numbers of rotamers available to the natural amino acids.

About this StructureAbout this Structure

3NUC is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

ReferenceReference

Mobile unnatural amino acid side chains in the core of staphylococcal nuclease., Wynn R, Harkins PC, Richards FM, Fox RO, Protein Sci. 1996 Jun;5(6):1026-31. PMID:8762134 Page seeded by OCA on Sun May 4 22:08:22 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:3nuc.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 3nuc |SIZE=350|CAPTION= <scene name='initialview01'>3nuc</scene>, resolution 1.9&Aring;
+The line below this paragraph, containing "STRUCTURE_3nuc", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PR3:S,S-PROPYLTHIOCYSTEINE'>PR3</scene>, <scene name='pdbligand=THP:THYMIDINE-3&#39;,5&#39;-DIPHOSPHATE'>THP</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Micrococcal_nuclease Micrococcal nuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.31.1 3.1.31.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_3nuc|  PDB=3nuc  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nuc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nuc OCA], [http://www.ebi.ac.uk/pdbsum/3nuc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3nuc RCSB]</span>
-}}
 '''STAPHLOCOCCAL NUCLEASE, 1-N-PROPANE THIOL DISULFIDE TO V23C VARIANT'''
@@ Line 30: / Line 27: @@
 [[Category: Richards, F M.]]
 [[Category: Wynn, R.]]
-[[Category: endonuclease]]
+[[Category: Endonuclease]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: nuclease]]
+[[Category: Nuclease]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 22:08:22 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:34:53 2008''