Electron cryomicroscopy: Difference between revisions
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Single-particle electron cryomicroscopy (cryo-EM) has become an important method for determining macromolecular structures. It is the basis for the [[Nobel Prizes for 3D Molecular Structure#2010-2019|2017 Nobel Prize in Chemistry]]. Although resolution is usually poorer than that obtained by [[X-ray crystallography]], cryo-EM has the great advantage of not requiring crystallization<ref>Obtaining highly-ordered crystals is perhaps the major obstacle to determination of structure by X-ray diffraction.</ref>. Cryo-EM is particularly | Single-particle electron cryomicroscopy (cryo-EM) has become an important method for determining macromolecular structures. It is the basis for the [[Nobel Prizes for 3D Molecular Structure#2010-2019|2017 Nobel Prize in Chemistry]]. Although resolution is usually poorer than that obtained by [[X-ray crystallography]], cryo-EM has the great advantage of not requiring crystallization<ref>Obtaining highly-ordered crystals is perhaps the major obstacle to determination of structure by X-ray diffraction.</ref>. Cryo-EM is particularly suited to determination of the structures of large complexes containing multiple proteins or nucleic acids, often the most difficult to crystallize. Early studies showed that docking of monomer crystal structures into even poor-resolution (e.g. 15 Å) cryo-EM maps of larger assemblies could reliably predict structure<ref>PMID: 10998630</ref>. | ||
* [https://www.youtube.com/watch?v=BJKkC0W-6Qk 3 min video] explaining the principles of cryo-EM. | * [https://www.youtube.com/watch?v=BJKkC0W-6Qk 3 min video] explaining the principles of cryo-EM. | ||