3cfb: Difference between revisions

Revision as of 21:42, 4 May 2008

High-resolution structure of blue fluorescent antibody EP2-19G2 in complex with stilbene hapten at 100K

OverviewOverview

The blue-emissive antibody EP2-19G2 that has been elicited against trans-stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge-transfer excited complex of an anionic stilbene and a cationic, parallel pi-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody.

About this StructureAbout this Structure

3CFB is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex., Debler EW, Kaufmann GF, Meijler MM, Heine A, Mee JM, Pljevaljcic G, Di Bilio AJ, Schultz PG, Millar DP, Janda KD, Wilson IA, Gray HB, Lerner RA, Science. 2008 Feb 29;319(5867):1232-5. PMID:18309081 Page seeded by OCA on Sun May 4 21:42:37 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:3cfb.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 3cfb |SIZE=350|CAPTION= <scene name='initialview01'>3cfb</scene>, resolution 1.60&Aring;
+The line below this paragraph, containing "STRUCTURE_3cfb", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Spb+Binding+Site+For+Residue+L+301'>AC1</scene>, <scene name='pdbsite=AC2:Spb+Binding+Site+For+Residue+B+302'>AC2</scene>, <scene name='pdbsite=AC3:Gol+Binding+Site+For+Residue+L+402'>AC3</scene>, <scene name='pdbsite=AC4:Gol+Binding+Site+For+Residue+L+406'>AC4</scene>, <scene name='pdbsite=AC5:Gol+Binding+Site+For+Residue+H+405'>AC5</scene>, <scene name='pdbsite=AC6:Gol+Binding+Site+For+Residue+A+401'>AC6</scene>, <scene name='pdbsite=AC7:Gol+Binding+Site+For+Residue+A+403'>AC7</scene> and <scene name='pdbsite=AC8:Gol+Binding+Site+For+Residue+A+404'>AC8</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SPB:4-(4-STYRYL-PHENYLCARBAMOYL)-BUTYRIC+ACID'>SPB</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_3cfb|  PDB=3cfb  |  SCENE=  }}
-|RELATEDENTRY=[[1fl3|1FL3]], [[1ub5|1UB5]], [[1ub6|1UB6]], [[3cfc|3CFC]], [[3cfd|3CFD]], [[3cfe|3CFE]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cfb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cfb OCA], [http://www.ebi.ac.uk/pdbsum/3cfb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3cfb RCSB]</span>
-}}
 '''High-resolution structure of blue fluorescent antibody EP2-19G2 in complex with stilbene hapten at 100K'''
@@ Line 27: / Line 24: @@
 [[Category: Debler, E W.]]
 [[Category: Wilson, I A.]]
-[[Category: blue-fluorescent antibody]]
+[[Category: Blue-fluorescent antibody]]
-[[Category: electron transfer]]
+[[Category: Electron transfer]]
-[[Category: hapten complex]]
+[[Category: Hapten complex]]
-[[Category: immune system]]
+[[Category: Immune system]]
-[[Category: immunoglobulin]]
+[[Category: Immunoglobulin]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 21:42:37 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:32:05 2008''