3bue: Difference between revisions

Revision as of 21:06, 4 May 2008

Crystal structure of the C-terminal domain hexamer of ArgR from Mycobacterium tuberculosis

OverviewOverview

The gene product of an open reading frame Rv1657 from Mycobacterium tuberculosis is a putative arginine repressor protein (ArgR), a transcriptional factor that regulates the expression of arginine-biosynthetic enzymes. Rv1657 was expressed and purified and a C-terminal domain was crystallized using the hanging-drop vapour-diffusion method. Diffraction data were collected and processed to a resolution of 2.15 A. The crystals belong to space group P1 and the Matthews coefficient suggests that the crystals contain six C-terminal domain molecules per unit cell. Previous structural and biochemical studies on the arginine repressor proteins from other organisms have likewise shown the presence of six molecules per unit cell.

About this StructureAbout this Structure

3BUE is a Single protein structure of sequence from Mycobacterium tuberculosis h37rv. Full crystallographic information is available from OCA.

ReferenceReference

Expression, purification and preliminary X-ray analysis of the C-terminal domain of an arginine repressor protein from Mycobacterium tuberculosis., Lu GJ, Garen CR, Cherney MM, Cherney LT, Lee C, James MN, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Nov 1;63(Pt, 11):936-9. Epub 2007 Oct 24. PMID:18007044 Page seeded by OCA on Sun May 4 21:06:53 2008

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OCA

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 [[Image:3bue.jpg|left|200px]]
- {{Structure
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-|GENE= argR, ahrC, Rv1657, MT1695, MTCY06H11.22 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 Mycobacterium tuberculosis H37Rv])
+-->
-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bue OCA], [http://www.ebi.ac.uk/pdbsum/3bue PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3bue RCSB]</span>
-}}
 '''Crystal structure of the C-terminal domain hexamer of ArgR from Mycobacterium tuberculosis'''
@@ Line 31: / Line 28: @@
 [[Category: Lu, G J.]]
 [[Category: TBSGC, TB Structural Genomics Consortium.]]
-[[Category: amino-acid biosynthesis]]
+[[Category: Amino-acid biosynthesis]]
-[[Category: arginine biosynthesis]]
+[[Category: Arginine biosynthesis]]
-[[Category: cytoplasm]]
+[[Category: Cytoplasm]]
-[[Category: dna binding protein]]
+[[Category: Dna binding protein]]
-[[Category: dna-binding]]
+[[Category: Dna-binding]]
-[[Category: hexamer]]
+[[Category: Hexamer]]
-[[Category: l-arginine binding domain]]
+[[Category: L-arginine binding domain]]
-[[Category: l-arginine repressor protein]]
+[[Category: L-arginine repressor protein]]
-[[Category: oligomerization domain]]
+[[Category: Oligomerization domain]]
-[[Category: repressor]]
+[[Category: Repressor]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: tb structural genomics consortium]]
+[[Category: Tb structural genomics consortium]]
-[[Category: tbsgc]]
+[[Category: Tbsgc]]
-[[Category: transcription]]
+[[Category: Transcription]]
-[[Category: transcription regulation]]
+[[Category: Transcription regulation]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 21:06:53 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:29:03 2008''