Sulfatase-modifying factor: Difference between revisions

<scene name='80/801257/Cv/2'>Glycosylated human sulfatase-modifying factor 1 complex with arylsulfatase peptide, Ca+2 ion and Cl- ion</scene>. The structure of SUMF1 complex with its substrate sulfatase terminal peptide CTPSR. <scene name='80/801257/Cv/3'>SUMF1 active site contains 2 cysteine residues and mutating either of them to serine results in an inactive enzyme</scene>. Cys341 was found to be <scene name='80/801257/Cv/4'>responsible for substrate binding and makes a Cys-Cys bond</scene> to the peptide cysteine residue. The peptide binds at the surface of SUMF1 in an extended conformation <scene name='80/801257/Cv/5'>making numerous interactions with the protein</scene><ref>PMID:16368756</ref>.

*<scene name='80/801257/Cv/6'>Cl coordination site</scene>.

*<scene name='80/801257/Cv/7'>1st Ca coordination site</scene>.

*<scene name='80/801257/Cv/8'>2nd Ca coordination site</scene>.