359d: Difference between revisions

Revision as of 20:17, 4 May 2008

INHIBITION OF THE HAMMERHEAD RIBOZYME CLEAVAGE REACTION BY SITE-SPECIFIC BINDING OF TB(III)

OverviewOverview

Terbium(III) [Tb(III)] was shown to inhibit the hammerhead ribozyme by competing with a single magnesium(II) ion. X-ray crystallography revealed that the Tb(III) ion binds to a site adjacent to an essential guanosine in the catalytic core of the ribozyme, approximately 10 angstroms from the cleavage site. Synthetic modifications near this binding site yielded an RNA substrate that was resistant to Tb(III) binding and capable of being cleaved, even in the presence of up to 20 micromolar Tb(III). It is suggested that the magnesium(II) ion thought to bind at this site may act as a switch, affecting the conformational changes required to achieve the transition state.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Inhibition of the hammerhead ribozyme cleavage reaction by site-specific binding of Tb., Feig AL, Scott WG, Uhlenbeck OC, Science. 1998 Jan 2;279(5347):81-4. PMID:9417029 Page seeded by OCA on Sun May 4 20:17:09 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:359d.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 359d |SIZE=350|CAPTION= <scene name='initialview01'>359d</scene>, resolution 2.900&Aring;
+The line below this paragraph, containing "STRUCTURE_359d", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=TB:TERBIUM(III)+ION'>TB</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_359d|  PDB=359d  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=359d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=359d OCA], [http://www.ebi.ac.uk/pdbsum/359d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=359d RCSB]</span>
-}}
 '''INHIBITION OF THE HAMMERHEAD RIBOZYME CLEAVAGE REACTION BY SITE-SPECIFIC BINDING OF TB(III)'''
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 ==About this Structure==
-D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=359D OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=359D OCA].
 ==Reference==
 Inhibition of the hammerhead ribozyme cleavage reaction by site-specific binding of Tb., Feig AL, Scott WG, Uhlenbeck OC, Science. 1998 Jan 2;279(5347):81-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9417029 9417029]
-[[Category: Protein complex]]
 [[Category: Feig, A L.]]
 [[Category: Scott, W G.]]
 [[Category: Uhlenbeck, O C.]]
-[[Category: catalytic rna]]
+[[Category: Catalytic rna]]
-[[Category: loop]]
+[[Category: Loop]]
-[[Category: rna hammerhead ribozyme]]
+[[Category: Rna hammerhead ribozyme]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 20:17:09 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:21:42 2008''