301d: Difference between revisions

Revision as of 20:14, 4 May 2008

CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MG(II)-SOAKED

OverviewOverview

The crystal structure of an unmodified hammerhead RNA in the absence of divalent metal ions has been solved, and it was shown that this ribozyme can cleave itself in the crystal when divalent metal ions are added. This biologically active RNA fold is the same as that found previously for two modified hammerhead ribozymes. Addition of divalent cations at low pH makes it possible to capture the uncleaved RNA in metal-bound form. A conformational intermediate, having an additional Mg(II) bound to the cleavage-site phosphate, was captured by freeze-trapping the RNA at an active pH prior to cleavage. The most significant conformational changes were limited to the active site of the ribozyme, and the changed conformation requires only small additional movements to reach a proposed transition-state.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Capturing the structure of a catalytic RNA intermediate: the hammerhead ribozyme., Scott WG, Murray JB, Arnold JR, Stoddard BL, Klug A, Science. 1996 Dec 20;274(5295):2065-9. PMID:8953035 Page seeded by OCA on Sun May 4 20:14:40 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:301d.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 301d |SIZE=350|CAPTION= <scene name='initialview01'>301d</scene>, resolution 3.000&Aring;
+The line below this paragraph, containing "STRUCTURE_301d", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_301d|  PDB=301d  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=301d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=301d OCA], [http://www.ebi.ac.uk/pdbsum/301d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=301d RCSB]</span>
-}}
 '''CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MG(II)-SOAKED'''
@@ Line 19: / Line 16: @@
 ==About this Structure==
-D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=301D OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=301D OCA].
 ==Reference==
 Capturing the structure of a catalytic RNA intermediate: the hammerhead ribozyme., Scott WG, Murray JB, Arnold JR, Stoddard BL, Klug A, Science. 1996 Dec 20;274(5295):2065-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8953035 8953035]
-[[Category: Protein complex]]
 [[Category: Arnold, J R.P.]]
 [[Category: Klug, A.]]
@@ Line 29: / Line 25: @@
 [[Category: Scott, W G.]]
 [[Category: Stoddard, B L.]]
-[[Category: catalytic rna]]
+[[Category: Catalytic rna]]
-[[Category: loop]]
+[[Category: Loop]]
-[[Category: rna hammerhead ribozyme]]
+[[Category: Rna hammerhead ribozyme]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 20:14:40 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:21:03 2008''