2z8d: Difference between revisions

Revision as of 20:02, 4 May 2008

The galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum in complex with lacto-N-biose

OverviewOverview

A recombinant galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) from Bifidobacterium longum JCM1217 has been prepared and crystallized by the hanging-drop vapour-diffusion method using 10 mg ml(-1) purified enzyme, 0.01 M zinc sulfate, 0.1 M MES buffer pH 5.9-6.4 and 20-22%(v/v) PEG MME 550 in the presence of 5 mM disaccharide ligands. Suitable crystals grew after 10 d incubation at 293 K. The crystals belong to space group C222(1), with unit-cell parameters a = 106.3, b = 143.6, c = 114.6 A for the lacto-N-biose I complex and a = 106.4, b = 143.4, c = 115.5 A for the galacto-N-biose complex, and diffracted to 1.85 and 1.99 A resolution, respectively.

About this StructureAbout this Structure

2Z8D is a Single protein structure of sequence from Bifidobacterium longum. Full crystallographic information is available from OCA.

ReferenceReference

Purification, crystallization and preliminary X-ray analysis of the galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum JCM1217., Wada J, Suzuki R, Fushinobu S, Kitaoka M, Wakagi T, Shoun H, Ashida H, Kumagai H, Katayama T, Yamamoto K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Sep 1;63(Pt, 9):751-3. Epub 2007 Aug 10. PMID:17768345 Page seeded by OCA on Sun May 4 20:02:10 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2z8d.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2z8d |SIZE=350|CAPTION= <scene name='initialview01'>2z8d</scene>, resolution 1.85&Aring;
+The line below this paragraph, containing "STRUCTURE_2z8d", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Nag+Binding+Site+For+Residue+A+2'>AC1</scene>, <scene name='pdbsite=AC2:Gal+Binding+Site+For+Residue+A+1'>AC2</scene>, <scene name='pdbsite=AC3:Nag+Binding+Site+For+Residue+B+2'>AC3</scene>, <scene name='pdbsite=AC4:Gal+Binding+Site+For+Residue+B+1'>AC4</scene>, <scene name='pdbsite=AC5:Zn+Binding+Site+For+Residue+A+3'>AC5</scene>, <scene name='pdbsite=AC6:Zn+Binding+Site+For+Residue+B+3'>AC6</scene>, <scene name='pdbsite=AC7:Zn+Binding+Site+For+Residue+A+4'>AC7</scene>, <scene name='pdbsite=AC8:Zn+Binding+Site+For+Residue+B+4'>AC8</scene>, <scene name='pdbsite=AC9:Zn+Binding+Site+For+Residue+B+5'>AC9</scene>, <scene name='pdbsite=BC1:Zn+Binding+Site+For+Residue+B+6'>BC1</scene>, <scene name='pdbsite=BC2:Zn+Binding+Site+For+Residue+B+7'>BC2</scene>, <scene name='pdbsite=BC3:Mes+Binding+Site+For+Residue+A+11'>BC3</scene> and <scene name='pdbsite=BC4:Mes+Binding+Site+For+Residue+B+12'>BC4</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= gltA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216816 Bifidobacterium longum])
+-->
-|DOMAIN=
+{{STRUCTURE_2z8d|  PDB=2z8d  |  SCENE=  }}
-|RELATEDENTRY=[[2z8e|2Z8E]], [[2z8f|2Z8F]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z8d OCA], [http://www.ebi.ac.uk/pdbsum/2z8d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2z8d RCSB]</span>
-}}
 '''The galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum in complex with lacto-N-biose'''
@@ Line 29: / Line 26: @@
 [[Category: Suzuki, R.]]
 [[Category: Wada, J.]]
-[[Category: abc transporter]]
+[[Category: Abc transporter]]
-[[Category: human milk oligosacchalide]]
+[[Category: Human milk oligosacchalide]]
-[[Category: mucin core-1]]
+[[Category: Mucin core-1]]
-[[Category: sugar binding protein]]
+[[Category: Sugar binding protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 20:02:10 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:19:22 2008''