2yyk: Difference between revisions

Revision as of 19:42, 4 May 2008

Crystal structure of the mutant of HpaB (T198I, A276G, and R466H)

OverviewOverview

The 4-hydroxyphenylacetate (4HPA) 3-monooxygenase is involved in the initial step of the 4HPA degradation pathway and catalyzes 4HPA hydroxylation to 3,4-dihydroxyphenylacetate. This enzyme consists of two components, an oxygenase (HpaB) and a reductase (HpaC). To understand the structural basis of the catalytic mechanism of HpaB, crystal structures of HpaB from Thermus thermophilus HB8 were determined in three states: a ligand-free form, a binary complex with FAD, and a ternary complex with FAD and 4HPA. Structural analysis revealed that the binding and dissociation of flavin are accompanied by conformational changes of the loop between beta5 and beta6 and of the loop between beta8 and beta9, leading to preformation of part of the substrate-binding site (Ser-197 and Thr-198). The latter loop further changes its conformation upon binding of 4HPA and obstructs the active site from the bulk solvent. Arg-100 is located adjacent to the putative oxygen-binding site and may be involved in the formation and stabilization of the C4a-hydroperoxyflavin intermediate.

About this StructureAbout this Structure

2YYK is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the oxygenase component (HpaB) of the 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8., Kim SH, Hisano T, Takeda K, Iwasaki W, Ebihara A, Miki K, J Biol Chem. 2007 Nov 9;282(45):33107-17. Epub 2007 Sep 5. PMID:17804419 Page seeded by OCA on Sun May 4 19:42:05 2008

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OCA

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 [[Image:2yyk.jpg|left|200px]]
- {{Structure
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/4-hydroxyphenylacetate_3-monooxygenase 4-hydroxyphenylacetate 3-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.3 1.14.13.3] </span>
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yyk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yyk OCA], [http://www.ebi.ac.uk/pdbsum/2yyk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2yyk RCSB]</span>
-}}
 '''Crystal structure of the mutant of HpaB (T198I, A276G, and R466H)'''
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 [[Category: Miki, K.]]
 [[Category: Takeda, K.]]
-[[Category: mutant]]
+[[Category: Mutant]]
-[[Category: oxidoreductase]]
+[[Category: Oxidoreductase]]
-[[Category: oxygnase component]]
+[[Category: Oxygnase component]]
-[[Category: riken spring-8 center]]
+[[Category: Riken spring-8 center]]
-[[Category: structurome]]
+[[Category: Structurome]]
-[[Category: two-component flavin diffusible monooxygenase]]
+[[Category: Two-component flavin diffusible monooxygenase]]
-[[Category: unligand form]]
+[[Category: Unligand form]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 19:42:05 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:16:39 2008''