2vf2: Difference between revisions

Revision as of 18:42, 4 May 2008

X-RAY CRYSTAL STRUCTURE OF HSAD FROM MYCOBACTERIUM TUBERCULOSIS

OverviewOverview

Tuberculosis is a major cause of death worldwide. Understanding of the pathogenicity of Mycobacterium tuberculosis has been advanced by gene analysis and has led to the identification of genes that are important for intracellular survival in macrophages. One of these genes encodes HsaD, a meta-cleavage product (MCP) hydrolase that catalyzes the hydrolytic cleavage of a carbon-carbon bond in cholesterol metabolism. This paper describes the production of HsaD as a recombinant protein and, following crystallization, the determination of its three-dimensional structure to 2.35 A resolution by X-ray crystallography at the Diamond Light Source in Oxfordshire, England. To the authors' knowledge, this study constitutes the first report of a structure determined at the new synchrotron facility. The volume of the active-site cleft of the HsaD enzyme is more than double the corresponding active-site volumes of related MCP hydrolases involved in the catabolism of aromatic compounds, consistent with the specificity of HsaD for steroids such as cholesterol. Knowledge of the structure of the enzyme facilitates the design of inhibitors.

About this StructureAbout this Structure

2VF2 is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

ReferenceReference

Structure of HsaD, a steroid-degrading hydrolase, from Mycobacterium tuberculosis., Lack N, Lowe ED, Liu J, Eltis LD, Noble ME, Sim E, Westwood IM, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jan 1;64(Pt 1):2-7., Epub 2007 Dec 20. PMID:18097091 Page seeded by OCA on Sun May 4 18:42:11 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2vf2.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2vf2 |SIZE=350|CAPTION= <scene name='initialview01'>2vf2</scene>, resolution 2.35&Aring;
+The line below this paragraph, containing "STRUCTURE_2vf2", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+B'>AC1</scene>, <scene name='pdbsite=AC2:Gol+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Gol+Binding+Site+For+Chain+B'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Chain+A'>AC4</scene> and <scene name='pdbsite=AC5:So4+Binding+Site+For+Chain+B'>AC5</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/2,6-dioxo-6-phenylhexa-3-enoate_hydrolase 2,6-dioxo-6-phenylhexa-3-enoate hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.8 3.7.1.8] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2vf2|  PDB=2vf2  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vf2 OCA], [http://www.ebi.ac.uk/pdbsum/2vf2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2vf2 RCSB]</span>
-}}
 '''X-RAY CRYSTAL STRUCTURE OF HSAD FROM MYCOBACTERIUM TUBERCULOSIS'''
@@ Line 33: / Line 30: @@
 [[Category: Sim, E.]]
 [[Category: Westwood, I M.]]
-[[Category: hsad]]
+[[Category: Hsad]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: meta-cleavage product hydrolase]]
+[[Category: Meta-cleavage product hydrolase]]
-[[Category: serine hydrolase]]
+[[Category: Serine hydrolase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 18:42:11 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:11:31 2008''