5yh4: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Miraculin-like protein from Vitis vinifera== | ==Miraculin-like protein from Vitis vinifera== | ||
<StructureSection load='5yh4' size='340' side='right' caption='[[5yh4]], [[Resolution|resolution]] 1.30Å' scene=''> | <StructureSection load='5yh4' size='340' side='right'caption='[[5yh4]], [[Resolution|resolution]] 1.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5yh4]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5yh4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vitis_vinifera Vitis vinifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YH4 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yh4 OCA], [https://pdbe.org/5yh4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yh4 RCSB], [https://www.ebi.ac.uk/pdbsum/5yh4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yh4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/D7TY99_VITVI D7TY99_VITVI] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 17: | Line 19: | ||
</div> | </div> | ||
<div class="pdbe-citations 5yh4" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5yh4" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Miraculin|Miraculin]] | |||
*[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Vitis vinifera]] | ||
[[Category: | [[Category: Furukawa N]] | ||
[[Category: | [[Category: Shimizu-Ibuka A]] | ||
Latest revision as of 11:29, 22 November 2023
Miraculin-like protein from Vitis viniferaMiraculin-like protein from Vitis vinifera
Structural highlights
FunctionPublication Abstract from PubMedThe so-called miraculin-like proteins (MLPs) are homologous to miraculin, a homodimeric protein with taste-modifying activity that converts sourness into sweetness. The identity between MLPs and miraculin generally ranges from 30% to 55%, and both MLPs and miraculin are categorized into the Kunitz-type soybean trypsin inhibitor (STI) family. MLP from grape (Vitis vinifera; vvMLP) exhibits significant homology to miraculin (61% identity), suggesting that vvMLP possesses miraculin-like properties. The results of size-exclusion chromatography and sensory analysis illustrated that vvMLP exists as a monomer in solution with no detectable taste-modifying activity. Crystal structure determination revealed that vvMLP exists as a beta-trefoil fold, similarly as other MLPs and Kunitz-type protein inhibitors. The conformation of the loops, including the so-called reactive loop in the STI family, was substantially different between vvMLP and STI. Recombinant vvMLP had inhibitory activity against trypsin (Ki=13.7muM), indicating that the protein can act as a moderate trypsin inhibitor. Structural and functional analysis of miraculin-like protein from Vitis vinifera.,Ohkura SI, Hori M, Saitoh K, Okuzawa T, Okamoto I, Furukawa N, Shimizu-Ibuka A Biochim Biophys Acta Proteins Proteom. 2018 Nov;1866(11):1125-1130. doi:, 10.1016/j.bbapap.2018.08.009. Epub 2018 Aug 27. PMID:30282610[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||||