5wjh: Difference between revisions
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==Using sound pulses to solve the crystal harvesting bottleneck== | ==Using sound pulses to solve the crystal harvesting bottleneck== | ||
<StructureSection load='5wjh' size='340' side='right' caption='[[5wjh]], [[Resolution|resolution]] 1.63Å' scene=''> | <StructureSection load='5wjh' size='340' side='right'caption='[[5wjh]], [[Resolution|resolution]] 1.63Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5wjh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Parengyodontium_album Parengyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJH FirstGlance]. <br> | <table><tr><td colspan='2'>[[5wjh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Parengyodontium_album Parengyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJH FirstGlance]. <br> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Parengyodontium album]] | [[Category: Parengyodontium album]] | ||
[[Category: Peptidase K]] | [[Category: Peptidase K]] | ||
Revision as of 11:12, 27 November 2019
Using sound pulses to solve the crystal harvesting bottleneckUsing sound pulses to solve the crystal harvesting bottleneck
Structural highlights
Function[PRTK_PARAQ] Hydrolyzes keratin at aromatic and hydrophobic residues. Publication Abstract from PubMedCrystal harvesting has proven to be difficult to automate and remains the rate-limiting step for many structure-determination and high-throughput screening projects. This has resulted in crystals being prepared more rapidly than they can be harvested for X-ray data collection. Fourth-generation synchrotrons will support extraordinarily rapid rates of data acquisition, putting further pressure on the crystal-harvesting bottleneck. Here, a simple solution is reported in which crystals can be acoustically harvested from slightly modified MiTeGen In Situ-1 crystallization plates. This technique uses an acoustic pulse to eject each crystal out of its crystallization well, through a short air column and onto a micro-mesh (improving on previous work, which required separately grown crystals to be transferred before harvesting). Crystals can be individually harvested or can be serially combined with a chemical library such as a fragment library. Using sound pulses to solve the crystal-harvesting bottleneck.,Samara YN, Brennan HM, McCarthy L, Bollard MT, Laspina D, Wlodek JM, Campos SL, Natarajan R, Gofron K, McSweeney S, Soares AS, Leroy L Acta Crystallogr D Struct Biol. 2018 Oct 1;74(Pt 10):986-999. doi:, 10.1107/S2059798318011506. Epub 2018 Oct 2. PMID:30289409[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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