2r6g: Difference between revisions

Revision as of 16:19, 4 May 2008

The Crystal Structure of the E. coli Maltose Transporter

OverviewOverview

The maltose uptake system of Escherichia coli is a well-characterized member of the ATP-binding cassette transporter superfamily. Here we present the 2.8-A crystal structure of the intact maltose transporter in complex with the maltose-binding protein, maltose and ATP. This structure, stabilized by a mutation that prevents ATP hydrolysis, captures the ATP-binding cassette dimer in a closed, ATP-bound conformation. Maltose is occluded within a solvent-filled cavity at the interface of the two transmembrane subunits, about halfway into the lipid bilayer. The binding protein docks onto the entrance of the cavity in an open conformation and serves as a cap to ensure unidirectional translocation of the sugar molecule. These results provide direct evidence for a concerted mechanism of transport in which solute is transferred from the binding protein to the transmembrane subunits when the cassette dimer closes to hydrolyse ATP.

About this StructureAbout this Structure

2R6G is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of a catalytic intermediate of the maltose transporter., Oldham ML, Khare D, Quiocho FA, Davidson AL, Chen J, Nature. 2007 Nov 22;450(7169):515-21. PMID:18033289 Page seeded by OCA on Sun May 4 16:19:32 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:2r6g.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2r6g |SIZE=350|CAPTION= <scene name='initialview01'>2r6g</scene>, resolution 2.80&Aring;
+The line below this paragraph, containing "STRUCTURE_2r6g", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ATP:ADENOSINE-5&#39;-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MAL:MALTOSE'>MAL</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Maltose-transporting_ATPase Maltose-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.19 3.6.3.19] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= malK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), malE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), malF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), malG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
+{{STRUCTURE_2r6g|  PDB=2r6g  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r6g OCA], [http://www.ebi.ac.uk/pdbsum/2r6g PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2r6g RCSB]</span>
-}}
 '''The Crystal Structure of the E. coli Maltose Transporter'''
@@ Line 31: / Line 28: @@
 [[Category: Oldham, M L]]
 [[Category: Quiocho, F A.]]
-[[Category: abc transporter]]
+[[Category: Abc transporter]]
-[[Category: atp binding cassette]]
+[[Category: Atp binding cassette]]
-[[Category: atp-binding]]
+[[Category: Atp-binding]]
-[[Category: catalytic intermediate]]
+[[Category: Catalytic intermediate]]
-[[Category: e. coli maltose transporter]]
+[[Category: E. coli maltose transporter]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: hydrolase/transport protein complex]]
+[[Category: Hydrolase/transport protein complex]]
-[[Category: inner membrane]]
+[[Category: Inner membrane]]
-[[Category: malk]]
+[[Category: Malk]]
-[[Category: maltodextrin binding protein]]
+[[Category: Maltodextrin binding protein]]
-[[Category: mbp]]
+[[Category: Mbp]]
-[[Category: membrane]]
+[[Category: Membrane]]
-[[Category: nucleotide-binding]]
+[[Category: Nucleotide-binding]]
-[[Category: periplasm]]
+[[Category: Periplasm]]
-[[Category: sugar transport]]
+[[Category: Sugar transport]]
-[[Category: transmembrane]]
+[[Category: Transmembrane]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 16:19:32 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:57:03 2008''