Garman lab: Interconversion of lysosomal enzyme specificities: Difference between revisions
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<text>NAGAL</text> | <text>NAGAL</text> | ||
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</jmol>). The active sites differ in only two residues, and the substrates bind in almost the same way (Fig 1 panel B and C insets: compare the active site of <scene name='78/786673/Gal_active_site/5'>GAL</scene> and < | </jmol>). The active sites differ in only two residues, and the substrates bind in almost the same way (Fig 1 panel B and C insets: compare the active site of <scene name='78/786673/Gal_active_site/5'>GAL</scene> and <jmol> | ||
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<script> model 2 | |||
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<text>NAGAL</text> | |||
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</jmol>). | |||
The researchers asked the following question: Is it possible to turn one enzyme into the other (in terms of reaction catalyzed)? Their hypothesis was that a simple swap of the two amino acids in the active site that are different would accomplish an interconversion of specificities. | The researchers asked the following question: Is it possible to turn one enzyme into the other (in terms of reaction catalyzed)? Their hypothesis was that a simple swap of the two amino acids in the active site that are different would accomplish an interconversion of specificities. | ||