2r1c: Difference between revisions

Revision as of 16:04, 4 May 2008

Coordinates of the thermus thermophilus ribosome binding factor A (RbfA) homology model as fitted into the CRYO-EM map of a 30S-RBFA complex

OverviewOverview

Ribosome binding factor A (RbfA) is a bacterial cold shock response protein, required for an efficient processing of the 5' end of the 16S ribosomal RNA (rRNA) during assembly of the small (30S) ribosomal subunit. Here we present a crystal structure of Thermus thermophilus (Tth) RbfA and a three-dimensional cryo-electron microscopic (EM) map of the Tth 30S*RbfA complex. RbfA binds to the 30S subunit in a position overlapping the binding sites of the A and P site tRNAs, and RbfA's functionally important C terminus extends toward the 5' end of the 16S rRNA. In the presence of RbfA, a portion of the 16S rRNA encompassing helix 44, which is known to be directly involved in mRNA decoding and tRNA binding, is displaced. These results shed light on the role played by RbfA during maturation of the 30S subunit, and also indicate how RbfA provides cells with a translational advantage under conditions of cold shock.

About this StructureAbout this Structure

2R1C is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Structural aspects of RbfA action during small ribosomal subunit assembly., Datta PP, Wilson DN, Kawazoe M, Swami NK, Kaminishi T, Sharma MR, Booth TM, Takemoto C, Fucini P, Yokoyama S, Agrawal RK, Mol Cell. 2007 Nov 9;28(3):434-45. PMID:17996707 Page seeded by OCA on Sun May 4 16:04:38 2008

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OCA

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 [[Image:2r1c.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2r1c |SIZE=350|CAPTION= <scene name='initialview01'>2r1c</scene>
+The line below this paragraph, containing "STRUCTURE_2r1c", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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+or leave the SCENE parameter empty for the default display.
-|GENE= TTHA0907 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])
+-->
-|DOMAIN=
+{{STRUCTURE_2r1c|  PDB=2r1c  |  SCENE=  }}
-|RELATEDENTRY=[[1jos|1JOS]], [[2dyj|2DYJ]], [[2r1g|2R1G]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r1c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r1c OCA], [http://www.ebi.ac.uk/pdbsum/2r1c PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2r1c RCSB]</span>
-}}
 '''Coordinates of the thermus thermophilus ribosome binding factor A (RbfA) homology model as fitted into the CRYO-EM map of a 30S-RBFA complex'''
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 [[Category: Wilson, D N.]]
 [[Category: Yokoyama, S.]]
-[[Category: cytoplasm]]
+[[Category: Cytoplasm]]
-[[Category: helix-kink-helix]]
+[[Category: Helix-kink-helix]]
-[[Category: kh domain]]
+[[Category: Kh domain]]
-[[Category: rna binding protein]]
+[[Category: Rna binding protein]]
-[[Category: rrna processing]]
+[[Category: Rrna processing]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 16:04:38 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:55:32 2008''