2bt1: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2bt1.gif|left|200px]]<br /> | [[Image:2bt1.gif|left|200px]]<br /><applet load="2bt1" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="2bt1" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="2bt1, resolution 2.70Å" /> | caption="2bt1, resolution 2.70Å" /> | ||
'''EPSTEIN BARR VIRUS DUTPASE IN COMPLEX WITH A,B-IMINO DUTP'''<br /> | '''EPSTEIN BARR VIRUS DUTPASE IN COMPLEX WITH A,B-IMINO DUTP'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
2BT1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_4 Human herpesvirus 4] with MG and DUP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] | 2BT1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_4 Human herpesvirus 4] with MG and DUP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] Known structural/functional Site: <scene name='pdbsite=AC1:Mg Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BT1 OCA]. | ||
==Reference== | ==Reference== | ||
| Line 31: | Line 30: | ||
[[Category: structural proteomics in europe]] | [[Category: structural proteomics in europe]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:57:47 2007'' | ||
Revision as of 19:48, 18 December 2007
|
EPSTEIN BARR VIRUS DUTPASE IN COMPLEX WITH A,B-IMINO DUTP
OverviewOverview
Deoxyuridine 5'-triphosphate pyrophosphatases (dUTPases) are ubiquitous, enzymes cleaving dUTP into dUMP and pyrophosphate. They occur as, monomeric, dimeric, or trimeric molecules. The trimeric and monomeric, enzymes both contain the same five characteristic sequence motifs but in a, different order, whereas the dimeric enzymes are not homologous. Monomeric, dUTPases only occur in herpesviruses, such as Epstein-Barr virus (EBV)., Here, we describe the crystal structures of EBV dUTPase in complex with, the product dUMP and a substrate analog alpha,beta-imino-dUTP. The, molecule consists of three domains forming one active site that has a, structure extremely similar to one of the three active sites of trimeric, dUTPases. The three domains functionally correspond to the subunits of the, trimeric form. Domains I and II have the dUTPase fold, but they differ, considerably in the regions that are not involved in the formation of the, unique active site, whereas domain III has only little secondary, structure.
About this StructureAbout this Structure
2BT1 is a Single protein structure of sequence from Human herpesvirus 4 with MG and DUP as ligands. Active as dUTP diphosphatase, with EC number 3.6.1.23 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
The monomeric dUTPase from Epstein-Barr virus mimics trimeric dUTPases., Tarbouriech N, Buisson M, Seigneurin JM, Cusack S, Burmeister WP, Structure. 2005 Sep;13(9):1299-310. PMID:16154087
Page seeded by OCA on Tue Dec 18 18:57:47 2007