2qca: Difference between revisions

Revision as of 14:42, 4 May 2008

A New Crystal Form of Bovine Pancreatic RNase A in Complex with 2'-Deoxyguanosine-5'-monophosphate

OverviewOverview

The structure of bovine pancreatic RNase A has been determined in complex with 2'-deoxyguanosine-5'-monophosphate (dGMP) at 1.33 A resolution at room temperature in a previously unreported unit cell belonging to space group P3(1). There are two molecules of nucleotide per enzyme molecule, one of which lies in the active-site cleft in the productive binding mode, whilst the guanine base of the other dGMP occupies the pyrimidine-specific binding site in a nonproductive mode such that it forms hydrogen bonds to the phosphate group of the first dGMP. This is the first RNase A structure containing a guanine base in the B2 binding site. Each dGMP molecule is involved in intermolecular interactions with adjacent RNase A molecules in the lattice and the two nucleotides appear to direct the formation of the crystal lattice. Because GMP may be produced during degradation of RNA, this association could represent an inhibitor complex and thereby affect the observed enzyme kinetics.

About this StructureAbout this Structure

2QCA is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

ReferenceReference

A new crystal form of bovine pancreatic RNase A in complex with 2'-deoxyguanosine-5'-monophosphate., Larson SB, Day JS, Cudney R, McPherson A, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Sep 1;63(Pt, 9):728-33. Epub 2007 Aug 31. PMID:17768339 Page seeded by OCA on Sun May 4 14:42:52 2008

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OCA

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 [[Image:2qca.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2qca |SIZE=350|CAPTION= <scene name='initialview01'>2qca</scene>, resolution 1.33&Aring;
+The line below this paragraph, containing "STRUCTURE_2qca", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DGP:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DGP</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00163 RNAse_Pc]</span>
+{{STRUCTURE_2qca|  PDB=2qca  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qca OCA], [http://www.ebi.ac.uk/pdbsum/2qca PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qca RCSB]</span>
-}}
 '''A New Crystal Form of Bovine Pancreatic RNase A in Complex with 2'-Deoxyguanosine-5'-monophosphate'''
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 [[Category: Larson, S B.]]
 [[Category: McPherson, A.]]
-[[Category: center for high-throughput structural biology]]
+[[Category: Center for high-throughput structural biology]]
-[[Category: chtsb]]
+[[Category: Chtsb]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: protein structure initiative]]
+[[Category: Protein structure initiative]]
-[[Category: protein-nucleotide complex]]
+[[Category: Protein-nucleotide complex]]
-[[Category: psi-2]]
+[[Category: Psi-2]]
-[[Category: ribonuclease]]
+[[Category: Ribonuclease]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 14:42:52 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:47:34 2008''