2vek: Difference between revisions
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==Structure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties== | ==Structure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties== | ||
<StructureSection load='2vek' size='340' side='right' caption='[[2vek]], [[Resolution|resolution]] 1.60Å' scene=''> | <StructureSection load='2vek' size='340' side='right'caption='[[2vek]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2vek]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trybb Trybb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VEK OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[2vek]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trybb Trybb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VEK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2VEK FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASF:3-(BUTYLSULPHONYL)-PROPANOIC+ACID'>ASF</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=TBU:TERTIARY-BUTYL+ALCOHOL'>TBU</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASF:3-(BUTYLSULPHONYL)-PROPANOIC+ACID'>ASF</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=TBU:TERTIARY-BUTYL+ALCOHOL'>TBU</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ag1|1ag1]], [[1dkw|1dkw]], [[1iig|1iig]], [[1ml1|1ml1]], [[1mss|1mss]], [[1mtm|1mtm]], [[1tpd|1tpd]], [[1tpe|1tpe]], [[1tri|1tri]], [[2v0t|2v0t]], [[2v5l|2v5l]], [[4tim|4tim]], [[5tim|5tim]], [[6tim|6tim]], [[1iih|1iih]], [[1kv5|1kv5]], [[1tpf|1tpf]], [[1trd|1trd]], [[1tsi|1tsi]], [[1tti|1tti]], [[1ttj|1ttj]], [[2j24|2j24]], [[2j27|2j27]], [[2v2c|2v2c]], [[2v2d|2v2d]], [[2v2h|2v2h]], [[3tim|3tim]], [[2vei|2vei]], [[2vel|2vel]], [[2vem|2vem]], [[2ven|2ven]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ag1|1ag1]], [[1dkw|1dkw]], [[1iig|1iig]], [[1ml1|1ml1]], [[1mss|1mss]], [[1mtm|1mtm]], [[1tpd|1tpd]], [[1tpe|1tpe]], [[1tri|1tri]], [[2v0t|2v0t]], [[2v5l|2v5l]], [[4tim|4tim]], [[5tim|5tim]], [[6tim|6tim]], [[1iih|1iih]], [[1kv5|1kv5]], [[1tpf|1tpf]], [[1trd|1trd]], [[1tsi|1tsi]], [[1tti|1tti]], [[1ttj|1ttj]], [[2j24|2j24]], [[2j27|2j27]], [[2v2c|2v2c]], [[2v2d|2v2d]], [[2v2h|2v2h]], [[3tim|3tim]], [[2vei|2vei]], [[2vel|2vel]], [[2vem|2vem]], [[2ven|2ven]]</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2vek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vek OCA], [http://pdbe.org/2vek PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vek RCSB], [http://www.ebi.ac.uk/pdbsum/2vek PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vek ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
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==See Also== | ==See Also== | ||
*[[Triose | *[[Triose phosphate isomerase 3D structures|Triose phosphate isomerase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Triose-phosphate isomerase]] | [[Category: Triose-phosphate isomerase]] | ||
[[Category: Trybb]] | [[Category: Trybb]] |