2bra: Difference between revisions
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[[Image:2bra.gif|left|200px]]<br /> | [[Image:2bra.gif|left|200px]]<br /><applet load="2bra" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="2bra" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="2bra, resolution 2.00Å" /> | caption="2bra, resolution 2.00Å" /> | ||
'''STRUCTURE OF N-TERMINAL FAD BINDING MOTIF OF MOUSE MICAL'''<br /> | '''STRUCTURE OF N-TERMINAL FAD BINDING MOTIF OF MOUSE MICAL'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2BRA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with CL and FAD as [http://en.wikipedia.org/wiki/ligands ligands]. | 2BRA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with CL and FAD as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Cl Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BRA OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: zinc]] | [[Category: zinc]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:56:08 2007'' | ||
Revision as of 19:46, 18 December 2007
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STRUCTURE OF N-TERMINAL FAD BINDING MOTIF OF MOUSE MICAL
OverviewOverview
During development, neurons are guided to their targets by short- and, long-range attractive and repulsive cues. MICAL, a large multidomain, protein, is required for the combined action of semaphorins and plexins in, axon guidance. Here, we present the structure of the N-terminal region of, MICAL (MICAL(fd)) determined by x-ray diffraction to 2.0 A resolution. The, structure shows that MICAL(fd) is an FAD-containing module structurally, similar to aromatic hydroxylases and amine oxidases. In addition, we, present biochemical data that show that MICAL(fd) is a flavoenzyme that in, the presence of NADPH reduces molecular oxygen to H(2)O(2) (K(m,NAPDH) =, 222 microM; k(cat) = 77 sec(-1)), a molecule with known signaling, properties. We propose that the H(2)O(2) produced by this reaction may be, one of the signaling molecules involved in axon guidance by MICAL.
About this StructureAbout this Structure
2BRA is a Single protein structure of sequence from Mus musculus with CL and FAD as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Structure and activity of the axon guidance protein MICAL., Nadella M, Bianchet MA, Gabelli SB, Barrila J, Amzel LM, Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16830-5. Epub 2005 Nov 7. PMID:16275926
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