2pgi: Difference between revisions

Revision as of 13:03, 4 May 2008

THE CRYSTAL STRUCTURE OF PHOSPHOGLUCOSE ISOMERASE-AN ENZYME WITH AUTOCRINE MOTILITY FACTOR ACTIVITY IN TUMOR CELLS

OverviewOverview

Phosphoglucose isomerase (PGI) plays a central role in both the glycolysis and the gluconeogenesis pathways. We present here the complete crystal structure of PGI from Bacillus stearothermophilus at 2.3-A resolution. We show that PGI has cell-motility-stimulating activity on mouse colon cancer cells similar to that of endogenous autocrine motility factor (AMF). PGI can also enhance neurite outgrowth on neuronal progenitor cells similar to that observed for neuroleukin. The results confirm that PGI is neuroleukin and AMF. PGI has an open twisted alpha/beta structural motif consisting of two globular domains and two protruding parts. Based on this substrate-free structure, together with the previously published biological, biochemical, and modeling results, we postulate a possible substrate-binding site that is located within the domains' interface for PGI and AMF. In addition, the structure provides evidence suggesting that the top part of the large domain together with one of the protruding loops might participate in inducing the neurotrophic activity.

About this StructureAbout this Structure

2PGI is a Single protein structure of sequence from Geobacillus stearothermophilus. The following page contains interesting information on the relation of 2PGI with [Aconitase and Iron Regulatory Protein 1]. Full crystallographic information is available from OCA.

ReferenceReference

The crystal structure of a multifunctional protein: phosphoglucose isomerase/autocrine motility factor/neuroleukin., Sun YJ, Chou CC, Chen WS, Wu RT, Meng M, Hsiao CD, Proc Natl Acad Sci U S A. 1999 May 11;96(10):5412-7. PMID:10318897 Page seeded by OCA on Sun May 4 13:03:50 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2pgi.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2pgi |SIZE=350|CAPTION= <scene name='initialview01'>2pgi</scene>, resolution 2.3&Aring;
+The line below this paragraph, containing "STRUCTURE_2pgi", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= PGIB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 Geobacillus stearothermophilus])
+-->
-|DOMAIN=
+{{STRUCTURE_2pgi|  PDB=2pgi  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pgi OCA], [http://www.ebi.ac.uk/pdbsum/2pgi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pgi RCSB]</span>
-}}
 '''THE CRYSTAL STRUCTURE OF PHOSPHOGLUCOSE ISOMERASE-AN ENZYME WITH AUTOCRINE MOTILITY FACTOR ACTIVITY IN TUMOR CELLS'''
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 [[Category: Meng, M.]]
 [[Category: Sun, Y J.]]
-[[Category: autocrinefactor]]
+[[Category: Autocrinefactor]]
-[[Category: crystallography]]
+[[Category: Crystallography]]
-[[Category: glycolysis]]
+[[Category: Glycolysis]]
-[[Category: motility]]
+[[Category: Motility]]
-[[Category: neuroleukin]]
+[[Category: Neuroleukin]]
-[[Category: phosphoglucose isomerase]]
+[[Category: Phosphoglucose isomerase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 13:03:50 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:35:36 2008''