6h02: Difference between revisions

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<StructureSection load='6h02' size='340' side='right' caption='[[6h02]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
<StructureSection load='6h02' size='340' side='right' caption='[[6h02]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6h02]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H02 FirstGlance]. <br>
<table><tr><td colspan='2'>[[6h02]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Lama Lama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H02 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h02 OCA], [http://pdbe.org/6h02 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h02 RCSB], [http://www.ebi.ac.uk/pdbsum/6h02 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h02 ProSAT]</span></td></tr>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MED23, ARC130, CRSP3, DRIP130, KIAA1216, SUR2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h02 OCA], [http://pdbe.org/6h02 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h02 RCSB], [http://www.ebi.ac.uk/pdbsum/6h02 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h02 ProSAT]</span></td></tr>
</table>
</table>
== Disease ==
== Disease ==
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<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
Sur2 is a metazoan Mediator subunit that interacts with the adenovirus E1A protein and functions in a mitogen-activated protein kinase pathway required for vulva development in Caenorhabditis elegans. We generated sur2-/- embryonic stem cells to analyze its function as a mammalian Mediator component. Our results show that Sur2 forms a subcomplex of the Mediator with two other subunits, TRAP/Med100 and 95. Knock-out of Sur2 prevents activation by E1A-CR3 and the mitogen-activated protein kinase-regulated ETS transcription factor Elk-1, but not by multiple other transcription factors. These results imply that specific activation domains stimulate transcription by binding to distinct Mediator subunits. Activation by E1A and Elk-1 requires recruitment of Mediator to a promoter by binding to its Sur2 subunit.
The Mediator complex transduces regulatory information from enhancers to promoters and performs essential roles in the initiation of transcription in eukaryotes. Human Mediator comprises 26 subunits forming three modules termed Head, Middle and Tail. Here we present the 2.8 A crystal structure of MED23, the largest subunit from the human Tail module. The structure identifies 25 HEAT repeats-like motifs organized into 5 alpha-solenoids. MED23 adopts an arch-shaped conformation, with an N-terminal domain (Nter) protruding from a large core region. In the core four solenoids, motifs wrap on themselves, creating triangular-shaped structural motifs on both faces of the arch, with extended grooves propagating through the interfaces between the solenoid motifs. MED23 is known to interact with several specific transcription activators and is involved in splicing, elongation, and post-transcriptional events. The structure rationalizes previous biochemical observations and paves the way for improved understanding of the cross-talk between Mediator and transcriptional activators.


Transcription control by E1A and MAP kinase pathway via Sur2 mediator subunit.,Stevens JL, Cantin GT, Wang G, Shevchenko A, Shevchenko A, Berk AJ Science. 2002 Apr 26;296(5568):755-8. Epub 2002 Apr 4. PMID:11934987<ref>PMID:11934987</ref>
Crystal structure of human Mediator subunit MED23.,Monte D, Clantin B, Dewitte F, Lens Z, Rucktooa P, Pardon E, Steyaert J, Verger A, Villeret V Nat Commun. 2018 Aug 23;9(1):3389. doi: 10.1038/s41467-018-05967-y. PMID:30140054<ref>PMID:30140054</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Human]]
[[Category: Lama]]
[[Category: Clantin, B]]
[[Category: Clantin, B]]
[[Category: Monte, D]]
[[Category: Monte, D]]
[[Category: Villeret, V]]
[[Category: Villeret, V]]
[[Category: Complex]]
[[Category: Complex]]
[[Category: Human]]
[[Category: Mediator]]
[[Category: Mediator]]
[[Category: Subunit]]
[[Category: Subunit]]
[[Category: Transcription]]
[[Category: Transcription]]

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