6a4x: Difference between revisions
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<StructureSection load='6a4x' size='340' side='right' caption='[[6a4x]], [[Resolution|resolution]] 1.63Å' scene=''> | <StructureSection load='6a4x' size='340' side='right' caption='[[6a4x]], [[Resolution|resolution]] 1.63Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6a4x]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A4X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A4X FirstGlance]. <br> | <table><tr><td colspan='2'>[[6a4x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_13385 Atcc 13385]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A4X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A4X FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AQI70_17615 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=146536 ATCC 13385])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a4x OCA], [http://pdbe.org/6a4x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a4x RCSB], [http://www.ebi.ac.uk/pdbsum/6a4x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a4x ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a4x OCA], [http://pdbe.org/6a4x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a4x RCSB], [http://www.ebi.ac.uk/pdbsum/6a4x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a4x ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Atcc 13385]] | |||
[[Category: Ge, H M]] | [[Category: Ge, H M]] | ||
[[Category: Wang, Y S]] | [[Category: Wang, Y S]] | ||
Revision as of 23:14, 19 September 2018
Oxidase ChaP-H2Oxidase ChaP-H2
Structural highlights
Publication Abstract from PubMedOxidative rearrangements play key roles in introducing structural complexity and biological activities of natural products biosynthesized by type II polyketide synthases (PKSs). Chartreusin (1) is a potent antitumor polyketide that contains a unique rearranged pentacyclic aromatic bilactone aglycone derived from a type II PKS. Herein, we report an unprecedented dioxygenase, ChaP, that catalyzes the final alpha-pyrone ring formation in 1 biosynthesis using flavin-activated oxygen as an oxidant. The X-ray crystal structures of ChaP and two homologues, docking studies, and site-directed mutagenesis provided insights into the molecular basis of the oxidative rearrangement that involves two successive C-C bond cleavage steps followed by lactonization. ChaP is the first example of a dioxygenase that requires a flavin-activated oxygen as a substrate despite lacking flavin binding sites, and represents a new class in the vicinal oxygen chelate enzyme superfamily. Molecular Basis for the Final Oxidative Rearrangement Steps in Chartreusin Biosynthesis.,Wang YS, Zhang B, Zhu J, Yang CL, Guo Y, Liu CL, Liu F, Huang H, Zhao S, Liang Y, Jiao RH, Tan RX, Ge HM J Am Chem Soc. 2018 Aug 15. doi: 10.1021/jacs.8b06623. PMID:30067334[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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